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Literature summary extracted from

  • Huang, K.; Takahara, S.; Kinouchi, T.; Takeyama, M.; Ishida, T.; Ueyama, H.; Nishi, K.; Ohkubo, I.
    Alanyl aminopeptidase from human seminal plasma: purification, characterization, and immunohistochemical localization in the male genital tract (1997), J. Biochem., 122, 779-787.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.11.2 resistant against several cycles of freezing and thawing Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline 100% inhibition at 1 mM Homo sapiens
3.4.11.2 actinonin 100% inhibition at 0.1 mM Homo sapiens
3.4.11.2 amastatin 100% inhibition at 0.1 mM Homo sapiens
3.4.11.2 arphamenine A 42% inhibition at 0.5 mM Homo sapiens
3.4.11.2 bestatin 96% inhibition at 0.1 mM Homo sapiens
3.4.11.2 Cd2+ 98% inhibition at 1 mM Homo sapiens
3.4.11.2 Co2+ 40% inhibition at 1 mM Homo sapiens
3.4.11.2 Cu2+ 96% inhibition at 1 mM Homo sapiens
3.4.11.2 EDTA 42% inhibition at 2 mM Homo sapiens
3.4.11.2 Hg2+ 71% inhibition at 1 mM Homo sapiens
3.4.11.2 leuhistin 100% inhibition at 0.1 mM Homo sapiens
3.4.11.2 Mg2+ not inhibitory at 1 mM Homo sapiens
3.4.11.2 Mn2+ 16% inhibition at 1 mM Homo sapiens
3.4.11.2 puromycin 67% inhibition at 0.1 mM Homo sapiens
3.4.11.2 Zn2+ 96% inhibition at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.1 0.33 amino acyl-4-methylcoumarin 7-amide Ala-, Met-, Tyr-, Leu-, Phe-, Arg-, Lys-, Gly-derivatives Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 137000 153000 light scattering, native PAGE, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 aminoacyl peptide Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 plasma
-
 Homo sapiens
-
3.4.11.2 seminal plasma
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.11.2 -30óC, at least four months, stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 aminoacyl peptide
-
 Homo sapiens ?
-
 ?
3.4.11.2 aminoacyl-4-methylcoumarin 7-amide + H2O Ala-, Met-, Tyr-, Leu-, Phe-, Arg-, Lys-, Gly-, Lys-Ala-, Arg-Arg- derivatives, not: Pro-, Pyr-, Gly-Pro-derivatives Homo sapiens amino acid + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 monomer 1 * 137000-153000, light scattering, native PAGE, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.2 50
-
 stable for at least 30 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7.5
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.2 6 10 stable Homo sapiens