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Literature summary extracted from

  • Midwinter R.G.; Pritchard, G.G.
    Aminopeptidase N from Streptococcus salivarius subsp. thermophilus NCDO 573: purification and properties (1994), J. Appl. Bacteriol., 77, 288-295.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline 100% inhibition at 1 mM Streptococcus salivarius
3.4.11.2 4-chloromercuribenzoate 100% inhibition at 1 mM Streptococcus salivarius
3.4.11.2 Co2+ 68% inhibition at 0.2 mM Streptococcus salivarius
3.4.11.2 Cu2+ 100% inhibition at 0.2 mM Streptococcus salivarius
3.4.11.2 EDTA 68% inhibition at 1 mM Streptococcus salivarius
3.4.11.2 iodoacetic acid 83% inhibition at 1 mM Streptococcus salivarius
3.4.11.2 Mg2+ not inhibitory at 0.2 mM Streptococcus salivarius
3.4.11.2 Mn2+ not inhibitory at 0.2 mM Streptococcus salivarius
3.4.11.2 Zn2+ 73% inhibition at 0.2 mM Streptococcus salivarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.025
-
 Leu-4-methylcoumarin 7-amide
-
 Streptococcus salivarius
3.4.11.2 0.06
-
 Lys-4-methylcoumarin 7-amide
-
 Streptococcus salivarius
3.4.11.2 0.09
-
 Arg-4-nitroanilide
-
 Streptococcus salivarius
3.4.11.2 0.11
-
 Phe-4-nitroanilide
-
 Streptococcus salivarius
3.4.11.2 0.17
-
 L-Leu-4-nitroanilide
-
 Streptococcus salivarius
3.4.11.2 0.2
-
 L-Lys-4-nitroanilide
-
 Streptococcus salivarius
3.4.11.2 1.4
-
 L-Ala-4-nitroanilide
-
 Streptococcus salivarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 98000
-
 SDS-PAGE Streptococcus salivarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 aminoacyl peptide Streptococcus salivarius
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Streptococcus salivarius
-
 subsp. thermophilus NCDO 573
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2
-
 Streptococcus salivarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.2 1.6 15 aminoacyl-4-nitroanilides as substrates Streptococcus salivarius
3.4.11.2 4
-
 Lys-4-methylcoumarin 7-amide as substrate Streptococcus salivarius
3.4.11.2 5.7
-
 Leu-4-methylcoumarin 7-amide as substrate Streptococcus salivarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 aminoacyl peptide
-
 Streptococcus salivarius ?
-
 ?
3.4.11.2 aminoacyl-p-nitroanilide + H2O
-
 Streptococcus salivarius amino acid + nitroaniline
-
 ?
3.4.11.2 dipeptides + H2O
-
 Streptococcus salivarius amino acids
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Arg-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Arg + 4-nitroaniline
-
 ?
3.4.11.2 L-Leu-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Leu + 4-nitroaniline
-
 ?
3.4.11.2 L-Lys-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Lys + 4-nitroaniline
-
 ?
3.4.11.2 L-Phe-4-nitroanilide + H2O
-
 Streptococcus salivarius L-Phe + 4-nitroaniline
-
 ?
3.4.11.2 Leu-4-methylcoumarin 7-amide + H2O
-
 Streptococcus salivarius Leu + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 Lys-4-methylcoumarin 7-amide + H2O
-
 Streptococcus salivarius Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 tripeptides + H2O
-
 Streptococcus salivarius dipeptides + amino acids
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 35
-
 substrate Lys-p-nitroanilide Streptococcus salivarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.2 10 65 low activity below and no activity above Streptococcus salivarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 6.9 7 substrate Lys-p-nitroanilide Streptococcus salivarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.2 5.8 7.6 substrate Lys-p-nitroanilide, greater than 50% of maximum activity between pH 5.8 and pH 7.6 Streptococcus salivarius