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Literature summary extracted from

  • Schnappauf, G.; Lipscomb, W.N.; Braus, G.H.
    Separation of inhibition and activation of the allosteric yeast chorismate mutase (1998), Proc. Natl. Acad. Sci. USA, 95, 2868-2873.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.5 L-Trp wild-type enzyme, mutant enzyme Ile225Thr/Thr226Ile and enzymes with mutations at Tyr234 are activated. No activation of mutant enzyme Thr226Ile Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 I225T mutant enzyme Ile225Thr is activated by Trp, but is insensitive to Tyr Saccharomyces cerevisiae
5.4.99.5 Y234F enzymes with mutations of Tyr234, especially Tyr234Phe are unresponsive to Tyr but are activated by Trp Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.5 L-Tyr wild-type enzyme is inhibited. Mutant enzymes with amino acid exchange at Thr234, especially Tyr234Phe, mutant enzyme Ile225Thr and Ile225Thr/Thr226Ile are insensitive Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.4
-
chorismate chorismate, mutant enzyme Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 0.4
-
chorismate wild-type enzyme, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 0.7
-
chorismate mutant enzyme Tyr234Phe, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 0.8
-
chorismate mutant enzyme Tyr234Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 1.3
-
chorismate mutant enzyme Tyr234Ser, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 1.5
-
chorismate mutant enzyme Ile225Thr/Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 1.6
-
chorismate mutant enzyme Glu23Asp, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 5.2
-
chorismate mutant enzyme Glu23Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 6
-
chorismate mutant enzyme Glu23Gln, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 6.3
-
chorismate mutant enzyme Tyr234Glu, activated by 0.01 mM Trp Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
Saccharomyces cerevisiae Prephenate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 14
-
chorismate mutant enzyme Glu23Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 44
-
chorismate mutant enzyme Tyr234Glu, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 74
-
chorismate mutant enzyme Tyr234Ser, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 171
-
chorismate mutant enzyme Glu23Gln, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 252
-
chorismate mutant enzyme Tyr234Ala, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 351
-
chorismate mutant enzyme Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 361
-
chorismate wild-type enzyme, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 535
-
chorismate mutant enzyme Ile225Thr/Thr226Ile, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 565
-
chorismate mutant enzyme Tyr234Phe, activated by 0.01 mM Trp Saccharomyces cerevisiae
5.4.99.5 625
-
chorismate mutant enzyme Glu23Asp, activated by 0.01 mM Trp Saccharomyces cerevisiae