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Literature summary extracted from

  • Zhang, S.; Pohnert, G.; Kongsaeree, P.; Wilson, D.B.; Clardy, J.; Ganem, B.
    Chorismate mutase-prephenate dehydratase from Escherichia coli. Study of catalytic and regulatory domains using genetically engineered proteins (1998), J. Biol. Chem., 273, 6248-6253.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.51 additional information expression of genes contaninig P-protein domains or subdomains, comparison of their catalytic activities and activation by phenylalanine Escherichia coli
5.4.99.5 additional information proteins containing residues 1-285 and residues 1-300 retain full chorismate mutase activity and prephenate dehydratase activity, but exhibit no feedback inhibition. Proteins containing residues 101-386 and residues 101-300 retain full prephenate dehydratase activity, but lack mutase activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.51 prephenate Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.51 Escherichia coli
-
-
-
5.4.99.5 Escherichia coli
-
 bifunctional enzyme chorismate mutase/prephenate dehydratase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.51 prephenate
-
 Escherichia coli phenylpyruvate + H2O + CO2
-
 ?
4.2.1.51 prephenate
-
 Escherichia coli ?
-
 ?
5.4.99.5 Chorismate
-
 Escherichia coli Prephenate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.5 54
-
 1 h, 50% loss of activity, wild-type enzyme Escherichia coli
5.4.99.5 58
-
 1 h, 50% inactivation, genetically engineered enzyme with amino acid residues 1-285 Escherichia coli
5.4.99.5 62
-
 1 h, 50% inactivation, genetically engineered enzyme with amino acid residues 1-300 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 39
-
 chorismate wild-type enzyme Escherichia coli
5.4.99.5 40.7
-
 chorismate genetically engineered enzyme containg the amino acid residues 1-300 Escherichia coli
5.4.99.5 44.3
-
 chorismate genetically engineered enzyme containg the amino acid residues 1-285 Escherichia coli