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Literature summary extracted from

  • Hertel, S.C.; Hieke, M.; Gröger, D.
    Purification and characterization of chorismate mutase isoenzymes from Ruta graveolens (1991), Acta Biotechnol., 11, 39-48.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.5 L-Trp enzyme form CM1 is activated Ruta graveolens
5.4.99.5 L-Trp enzyme form CM2 is unaffected Ruta graveolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.5 4-Methyl-DL-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
5.4.99.5 6-Methyl-DL-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
5.4.99.5 Ba2+
-
 Ruta graveolens
5.4.99.5 Co2+
-
 Ruta graveolens
5.4.99.5 Cu2+ strong Ruta graveolens
5.4.99.5 DL-3-fluoro-Phe enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
5.4.99.5 DL-5-Fluoro-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
5.4.99.5 DL-5-hydroxy-Trp enzyme form CM1 is inhibited, enzyme form CM2 not Ruta graveolens
5.4.99.5 Fe2+
-
 Ruta graveolens
5.4.99.5 Hg2+ HgCl2 Ruta graveolens
5.4.99.5 L-Phe
-
 Escherichia coli
5.4.99.5 L-Phe enzyme form CM1 is inhibited; Trp reverses inhibition Ruta graveolens
5.4.99.5 L-Tyr
-
 Escherichia coli
5.4.99.5 L-Tyr enzyme form CM1 is inhibited; enzyme form CM2 is not inhibited; Trp reverses inhibition Ruta graveolens
5.4.99.5 Mn2+
-
 Ruta graveolens
5.4.99.5 Pb2+
-
 Ruta graveolens
5.4.99.5 PCMB
-
 Ruta graveolens
5.4.99.5 Zn2+ strong Ruta graveolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.5
-
 chorismate enzyme form CM2 Ruta graveolens
5.4.99.5 1.1
-
 chorismate enzyme CM1 Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 45000
-
 enzyme form CM2, gel filtration Ruta graveolens
5.4.99.5 56000
-
 enzyme form CM1, gel filtration Ruta graveolens

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Escherichia coli
-
-
-
5.4.99.5 Ruta graveolens
-
 2 isoenzymes: CM1 and CM2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5 partial Ruta graveolens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.5 cell suspension culture
-
 Ruta graveolens
-

Storage Stability

EC Number Storage Stability Organism
5.4.99.5 -20°C, enzyme form CM1 and CM2 are stable Ruta graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Escherichia coli Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Ruta graveolens Prephenate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.5 35 37 enzyme form CM1 Ruta graveolens
5.4.99.5 56
-
 enzyme form CM2 Ruta graveolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.5 45
-
 5 min, enzyme form CM1, 50% loss of activity Ruta graveolens
5.4.99.5 50 60 5 min, enzyme form CM2, stable Ruta graveolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.5 6 10 enzyme form CM2 Ruta graveolens
5.4.99.5 7.8
-
 enzyme form CM1 Ruta graveolens