Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gray, J.V.; Golinelli-Pimpaneau, B.; Knowles, J.R.
    Monofunctional chorismate mutase from Bacillus subtilis: purification of the protein, molecular cloning of the gene, and overexpression of the gene product in Escherichia coli (1990), Biochemistry, 29, 376-383.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expression in Escherichia coli Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.1
-
 chorismate
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 14500
-
 2 * 14500, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Bacillus subtilis
-
 monofunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.5 200
-
-
 Bacillus subtilis
5.4.99.5 210
-
 recombinant enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Bacillus subtilis Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 dimer 2 * 14500, SDS-PAGE Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 50
-
 chorismate
-
 Bacillus subtilis