EC Number | General Stability | Organism |
---|---|---|
1.3.1.12 | citrate stabilizes | Escherichia coli |
1.3.1.12 | glycerol stabilizes | Escherichia coli |
1.3.1.12 | NAD+ plus tyrosine stabilizes | Escherichia coli |
1.3.1.12 | thiols stabilize | Escherichia coli |
5.4.99.5 | the enzyme is stable in high concentrations of glycerol, above 10% v/v, pH 7.5-8.0, and in the presence of thiols, citrate, and NAD+ | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.12 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
1.3.1.12 | iodoacetamide | - |
Escherichia coli | |
1.3.1.12 | L-tyrosine | - |
Escherichia coli | |
5.4.99.5 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
5.4.99.5 | iodoacetamide | - |
Escherichia coli | |
5.4.99.5 | L-Tyr | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.12 | 0.045 | 0.09 | prephenate | - |
Escherichia coli | |
1.3.1.12 | 0.13 | 0.15 | NAD+ | - |
Escherichia coli | |
5.4.99.5 | 0.04 | - |
chorismate | in presence of NAD+ | Escherichia coli | |
5.4.99.5 | 0.092 | - |
chorismate | pH 7.5, in absence of NAD+ | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.12 | 78000 | 100000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, sedimentation equilibrium centrifugation | Escherichia coli |
5.4.99.5 | 39000 | - |
2 * 39000, SDS-PAGE | Escherichia coli |
5.4.99.5 | 42042 | - |
2 * 42042, calculation from nucleotide sequence | Escherichia coli |
5.4.99.5 | 78000 | - |
sedimentation equilibrium analysis | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.12 | prephenate + NAD+ | Escherichia coli | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.12 | Escherichia coli | - |
- |
- |
5.4.99.5 | Escherichia coli | - |
bifunctional enzyme chorismate mutase/prephenate dehydratase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.12 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Escherichia coli |
5.4.99.5 | chorismate mutase/prephenate dehydratase | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.12 | 52 | 101 | - |
Escherichia coli |
5.4.99.5 | additional information | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.12 | prephenate + NAD+ | - |
Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
1.3.1.12 | prephenate + NAD+ | biosynthesis of L-tyrosine | Escherichia coli | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
5.4.99.5 | Chorismate | - |
Escherichia coli | Prephenate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.12 | dimer | 2 * 39000-42000, SDS-PAGE | Escherichia coli |
5.4.99.5 | dimer | 2 * 39000, SDS-PAGE | Escherichia coli |
5.4.99.5 | dimer | 2 * 42042, calculation from nucleotide sequence | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.12 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.5 | NAD+ | enhances activity | Escherichia coli |