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Literature summary extracted from

  • Raux, E.; Schubert, H.L.; Woodcock, S.C.; Wilson, K.S.; Warren, M.J.
    Cobalamin (vitamin B12) biosynthesis--cloning, expression and crystallisation of the Bacillus megaterium S-adenosyl-L-methionine-dependent cobalt-precorrin-4 transmethylase CbiF (1998), Eur. J. Biochem., 254, 341-346.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.271 expression in Escherichia coli Priestia megaterium
2.1.1.271 overexpression in Escherichia coli Priestia megaterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.271
-
 Priestia megaterium
2.1.1.271 to 2.4 A resolution. Protein shows two discrete crystal forms, and crystals only grow in the presence of either S-adenosyl-L-methionine or S-adenosyl-L-homocysteine Priestia megaterium

General Stability

EC Number General Stability Organism
2.1.1.271 stable to freezing at -20°C Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.271 31000
-
 2 * 31000, SDS-PAGE Priestia megaterium
2.1.1.271 31000
-
 2 * 31000, SDS-PAGE, recombinant protein including His-tag Priestia megaterium
2.1.1.271 62000
-
 gel filtration Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.271 Priestia megaterium
-
-
-
2.1.1.271 Priestia megaterium O87696
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.271
-
 Priestia megaterium
2.1.1.271 recombinant enzyme Priestia megaterium

Storage Stability

EC Number Storage Stability Organism
2.1.1.271 -20°C, protein reduced in volume to a concentration in excess of 10 mg/ml is stable to freezing and remains in solution when thawed Priestia megaterium
2.1.1.271 4°C, protein can be stored for several weeks Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.271 S-adenosyl-L-methionine + cobalt-precorrin-4
-
 Priestia megaterium S-adenosyl-L-homocysteine + cobalt-precorrin-5
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.271 dimer 2 * 31000, SDS-PAGE Priestia megaterium
2.1.1.271 dimer 2 * 31000, SDS-PAGE, recombinant protein including His-tag Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
2.1.1.271 CbiF
-
 Priestia megaterium
2.1.1.271 Cobalt-precorrin-4 C(11)-methyltransferase
-
 Priestia megaterium
2.1.1.271 cobalt-precorrin-4 transmethylase
-
 Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.271 S-adenosyl-L-methionine cofactor, but protein does not associate with S-adenosyl-L-methionine upon gel filtration Priestia megaterium

General Information

EC Number General Information Comment Organism
2.1.1.271 physiological function His-tagged protein is able to complement a Salmonella typhimurium cbiF mutant Priestia megaterium