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Literature summary extracted from
- Source of methylmalonyl-coenzyme A for erythromycin synthesis: methylmalonyl-coenzyme A mutase from Streptomyces erythreus (1984), Antimicrob. Agents Chemother., 25, 173-178.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.2 | 0.31 | - |
(R)-2-methyl-3-oxopropanoyl-CoA | - |
Saccharopolyspora erythraea |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 63000 | - |
x * 63000, SDS-PAGE | Saccharopolyspora erythraea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | Succinyl-CoA | Saccharopolyspora erythraea | succinyl-CoA is isomerized to methylmalonyl-CoA before incorporation into the macrocyclic lactone of erythromycin | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | Saccharopolyspora erythraea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | partial | Saccharopolyspora erythraea |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 0.47 | - |
- |
Saccharopolyspora erythraea |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.2 | -10°C, 50 mM Tris/HCl buffer, pH 8.0, 1 mM dithiothreitol, about 20-30% loss of activity after 2 days | Saccharopolyspora erythraea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Saccharopolyspora erythraea | ? | - |
? | |
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Saccharopolyspora erythraea | succinyl-CoA | - |
? | |
| 5.4.99.2 | Succinyl-CoA | succinyl-CoA is isomerized to methylmalonyl-CoA before incorporation into the macrocyclic lactone of erythromycin | Saccharopolyspora erythraea | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | ? | x * 63000, SDS-PAGE | Saccharopolyspora erythraea |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 7.5 | - |
- |
Saccharopolyspora erythraea |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | cobamide | Km: 0.00009 mM | Saccharopolyspora erythraea | |
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Release 2023.1 (January 2023)
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