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Literature summary extracted from

  • Szilagyi, C.M.; Sarfati, P.; Pradayrol, L.; Morisset, J.
    Purification, characterization and substrate specificity of rat pancreatic elastase II (1995), Biochim. Biophys. Acta, 1251, 55-65.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.71 cDNA clone isolated Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.36 3,4-dichloroisocoumarin
-
Rattus norvegicus
3.4.21.36 Soybean trypsin inhibitor
-
Rattus norvegicus
3.4.21.71 3,4-dichloroisocoumarin causes total inhibition Rattus norvegicus
3.4.21.71 diisopropyl fluorophosphate causes total inhibition Rattus norvegicus
3.4.21.71 additional information enzyme activity is not affected by TPCK nor by phosphoramidon Rattus norvegicus
3.4.21.71 N-alpha-tosyl-L-lysine-chloromethyl ketone most potent inhibitor Rattus norvegicus
3.4.21.71 phenylmethylsulfonyl fluoride 1 mM, 50% inhibition Rattus norvegicus
3.4.21.71 Soybean trypsin inhibitor most potent inhibitor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.71 0.089
-
Somatostatin-14 pH 8.0, 25°C Rattus norvegicus
3.4.21.71 1.567
-
Suc-Ala-Ala-Pro-Met-p-nitroanilide pH 8.0, 25°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.71 additional information increasing Ca2+ activity has no effect on elastase activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.36 29000
-
gel filtration Rattus norvegicus
3.4.21.71 27260
-
predicted from cDNA clone Rattus norvegicus
3.4.21.71 29000
-
SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.71 Somatostatin-14 + H2O Rattus norvegicus hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.36 Rattus norvegicus
-
-
-
3.4.21.71 Rattus norvegicus
-
rat, male Wistar
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.36 no modification no carbohydrate Rattus norvegicus
3.4.21.71 additional information no carbohydrate moiety detected in the structure Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.36
-
Rattus norvegicus
3.4.21.71
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.36 pancreas
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.36 additional information
-
-
Rattus norvegicus
3.4.21.71 additional information
-
specific activity 118.83, activity of the enzyme is determined by its ability to degrade 125I-S-14, ng degraded/mg protein Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.36 somatostatin 14 + H2O
-
Rattus norvegicus ?
-
?
3.4.21.36 succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
-
Rattus norvegicus ?
-
?
3.4.21.71 additional information elastin-Congo red is no substrate Rattus norvegicus ?
-
?
3.4.21.71 SMS 201-995 + H2O
-
Rattus norvegicus ?
-
?
3.4.21.71 Somatostatin-14 + H2O
-
Rattus norvegicus ?
-
?
3.4.21.71 Somatostatin-14 + H2O hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract Rattus norvegicus ?
-
?
3.4.21.71 Suc-Ala-Ala-Pro-Met-p-nitroanilide + H2O
-
Rattus norvegicus p-nitroaniline + Suc-Ala-Ala-Pro-Met
-
?
3.4.21.71 Suc-Ala-Ala-Val-Ala-p-nitroanilide + H2O
-
Rattus norvegicus p-nitroaniline + Suc-Ala-Ala-Val-Ala
-
?
3.4.21.71 Suc-Tyr-Leu-Val-p-nitroanilide + H2O
-
Rattus norvegicus p-nitroaniline + Suc-Tyr-Leu-Val
-
?

Subunits

EC Number Subunits Comment Organism
3.4.21.36 monomer 1 * 29000, SDS-PAGE Rattus norvegicus
3.4.21.71 monomer 1 * 29000, SDS-PAGE Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.71 5 9
-
Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.71 Rattus norvegicus isoelectric focusing 5.9 5.6