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Literature summary extracted from
- Purification and characterization of chicken liver cathepsin B (1988), J. Biochem., 104, 472-476.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | antipain | 93% inhibition at 1 microM | Gallus gallus |  |
| 3.4.22.1 | chymostatin | 85% inhibition at 5 microM | Gallus gallus | |
| 3.4.22.1 | iodoacetic acid | 100% inhibition at 0.5 mM | Gallus gallus | |
| 3.4.22.1 | leupeptin | 98% inhibition at 1 microM | Gallus gallus | |
| 3.4.22.1 | phenylmethanesulfonyl fluoride | no inhibition at 0.1 mM | Gallus gallus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 0.2 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | - |
Gallus gallus | |
| 3.4.22.1 | 20 | - |
benzoyl-DL-Arg-p-nitroanilide | - |
Gallus gallus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 24500 | - |
1 * 24500 or 25500 + 1 * 5000, SDS-PAGE | Gallus gallus |
| 3.4.22.1 | 28000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | Proteins + H2O | Gallus gallus | turnover of intracellular proteins | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | Gallus gallus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.1 | - |
Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | liver | - |
Gallus gallus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.22.1 | -80°C, pH 7.5, 3 months, stable | Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Gallus gallus | benzoyl-DL-Arg + p-nitroaniline | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide | - |
Gallus gallus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.1 | Proteins + H2O | turnover of intracellular proteins | Gallus gallus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.22.1 | dimer | 1 * 24500 or 25500 + 1 * 5000, SDS-PAGE | Gallus gallus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 2.4 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | - |
Gallus gallus | |
| 3.4.22.1 | 83 | - |
benzoyl-DL-Arg-p-nitroanilide | - |
Gallus gallus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 6.2 | - |
- |
Gallus gallus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 5.5 | 7 | - |
Gallus gallus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 4 | 7.5 | stable | Gallus gallus |
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