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Literature summary extracted from
- A comparison of four cathepsins (B, L, N and S) with collagenolytic activity from rabbit spleen (1988), Biochem. J., 256, 433-440.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | cysteine | activates | Oryctolagus cuniculus |  |
| 3.4.22.1 | thiol compounds | - |
Oryctolagus cuniculus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | cystatin | poor inhibitor, 51% inhibition at 100 nM | Oryctolagus cuniculus | |
| 3.4.22.15 | Benzyloxycarbonyl-Phe-Phe-CHN2 | - |
Oryctolagus cuniculus | |
| 3.4.22.15 | Cys | - |
Oryctolagus cuniculus | |
| 3.4.22.15 | cystatin | - |
Oryctolagus cuniculus | |
| 3.4.22.15 | L-3-carboxy-trans-2,3-epoxypropionyl-leucylamido-(3-methyl)butane | - |
Oryctolagus cuniculus | |
| 3.4.22.27 | Benzyloxycarbonyl-Phe-Phe-CHN2 | - |
Oryctolagus cuniculus | |
| 3.4.22.27 | cystatin | - |
Oryctolagus cuniculus | |
| 3.4.22.27 | Ep-475 | 0.01 mM, complete inhibition | Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 25250 | - |
gel filtration | Oryctolagus cuniculus |
| 3.4.22.15 | 23500 | - |
gel filtration | Oryctolagus cuniculus |
| 3.4.22.27 | 30000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | Collagen + H2O | Oryctolagus cuniculus | - |
? | - |
? | |
| 3.4.22.1 | Proteins + H2O | Oryctolagus cuniculus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | Oryctolagus cuniculus | - |
- |
- |
| 3.4.22.15 | Oryctolagus cuniculus | - |
- |
- |
| 3.4.22.27 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.1 | - |
Oryctolagus cuniculus |
| 3.4.22.27 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | spleen | - |
Oryctolagus cuniculus | - |
| 3.4.22.15 | spleen | - |
Oryctolagus cuniculus | - |
| 3.4.22.27 | spleen | - |
Oryctolagus cuniculus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.22.1 | 4°C, sodium acetate buffer, pH 5.0, several months, stable | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | azocasein + H2O | - |
Oryctolagus cuniculus | fragments of azocasein | - |
? | |
| 3.4.22.1 | benzoyl-Phe-Val-Arg-4-methylcoumarin-7-amide + H2O | - |
Oryctolagus cuniculus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide | - |
Oryctolagus cuniculus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.1 | Collagen + H2O | - |
Oryctolagus cuniculus | Hydrolyzed collagen | - |
? | |
| 3.4.22.1 | Collagen + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.1 | gelatin + H2O | - |
Oryctolagus cuniculus | hydrolyzed gelatine | - |
? | |
| 3.4.22.1 | Proteins + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.15 | azocasein + H2O | low activity | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.15 | benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.15 | benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.15 | benzyloxycarbonyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.15 | Collagen + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.15 | Gelatin + H2O | low activity | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.27 | Arg-7-amido-4-methylcoumarin + H2O | no activity detected | Oryctolagus cuniculus | Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.27 | azocasein + H2O | reaction only in presence of urea | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.27 | benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Oryctolagus cuniculus | benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.27 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Oryctolagus cuniculus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.22.27 | Collagen + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.27 | Gelatin + H2O | alpha-chain | Oryctolagus cuniculus | ? | - |
? | |
| 3.4.22.27 | additional information | no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and benzoyl-Gly-Gly-7-amido-4-methylcoumarin | Oryctolagus cuniculus | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 0.000000001 | - |
azocasein | - |
Oryctolagus cuniculus | |
| 3.4.22.1 | 0.00000000817 | - |
gelatin | - |
Oryctolagus cuniculus | |
| 3.4.22.1 | 0.000625 | - |
Collagen | - |
Oryctolagus cuniculus | |
| 3.4.22.1 | 3.45 | - |
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide | - |
Oryctolagus cuniculus | |
| 3.4.22.1 | 7.67 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | - |
Oryctolagus cuniculus | |
| 3.4.22.1 | 8.12 | - |
benzoyl-Phe-Val-Arg-4-methylcoumaryl-7-amide | - |
Oryctolagus cuniculus | |
| 3.4.22.27 | 0.000000000583 | - |
gelatin | alpha-chain | Oryctolagus cuniculus | |
| 3.4.22.27 | 0.000000011 | - |
azocasein | in presence of urea | Oryctolagus cuniculus | |
| 3.4.22.27 | 0.000000548 | - |
Collagen | alpha-chain | Oryctolagus cuniculus | |
| 3.4.22.27 | 0.02 | - |
benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin | - |
Oryctolagus cuniculus | |
| 3.4.22.27 | 0.0283 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Oryctolagus cuniculus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.22.27 | Oryctolagus cuniculus | isoelectric focusing | - |
6.8 |
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