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Literature summary extracted from

  • Tezuka, T.; Tonomura, K.
    Purification and properties of an enzyme catalyzing the splitting of carbon-mercury linkages from mercury-resistant Pseudomonas K-62 strain. I. Splitting enzyme 1 (1976), J. Biochem., 80, 79-87.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.99.1.2 inactivated by freezing Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.99.1.2 0.053
-
p-chloromercuribenzoic acid
-
Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.99.1.2 19000
-
gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.2 Pseudomonas sp.
-
K-62
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.2
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.99.1.2 additional information
-
-
Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.99.1.2 stable for at least 3 months, pH 7.5, 5% glycerol, 2°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.2 ethylmercuric chloride + H+
-
Pseudomonas sp. C2H6 + Hg2+ + Cl-
-
?
4.99.1.2 methylmercuric chloride + H+
-
Pseudomonas sp. CH4 + Hg2+ + Cl-
-
?
4.99.1.2 additional information
-
Pseudomonas sp. ?
-
?
4.99.1.2 p-chloromercuribenzoate
-
Pseudomonas sp. Hg2+ + p-hydroxybenzoate
-
?
4.99.1.2 phenylmercuric acetate + H+
-
Pseudomonas sp. benzene + Hg+ + Cl-
-
?
4.99.1.2 RHg+ + H+
-
Pseudomonas sp. RH + Hg2+
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.99.1.2 50
-
-
Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.99.1.2 30 60
-
Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.99.1.2 7
-
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.99.1.2 5.5 9
-
Pseudomonas sp.