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Literature summary extracted from

  • Begley, T.P.; Walts, A.E.; Walsh, C.T.
    Bacterial organomercurial lyase: overproduction, isolation, and characterization (1986), Biochemistry, 25, 7186-7192.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.99.1.2 additional information strong substrate inhibition above 1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.99.1.2 0.5
-
 methyl mercuric chloride
-
 Escherichia coli
4.99.1.2 0.8
-
 sec-butyl mercuric bromide
-
 Escherichia coli
4.99.1.2 0.8
-
 tert-butyl mercuric chloride
-
 Escherichia coli
4.99.1.2 0.9
-
 phenyl mercuric acetate
-
 Escherichia coli
4.99.1.2 0.9
-
 n-butyl mercuric chloride
-
 Escherichia coli
4.99.1.2 1
-
 vinylmercuric bromide
-
 Escherichia coli
4.99.1.2 1.3
-
 crotonyl mercuric bromide
-
 Escherichia coli
4.99.1.2 3.3
-
 cis-2-butenylmercuric chloride
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.99.1.2 additional information no associated metals Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.99.1.2 22400
-
 SDS-PAGE Escherichia coli
4.99.1.2 32000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.2 organomercurial salts Escherichia coli microbial detoxification ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.2
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.99.1.2 0.06
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.2 cis-2-butenylmercuric chloride + H+
-
 Escherichia coli ?
-
 ?
4.99.1.2 crotonyl mercuric bromide + H+
-
 Escherichia coli crotonaldehyde + Hg2+ + Br-
-
 ?
4.99.1.2 methyl mercuric chloride + H+
-
 Escherichia coli CH4 + Hg2+ + Cl-
-
 ?
4.99.1.2 additional information
-
 Escherichia coli ?
-
 ?
4.99.1.2 n-butyl mercuric chloride + H+
-
 Escherichia coli ?
-
 ?
4.99.1.2 organomercurial salts microbial detoxification Escherichia coli ?
-
 ?
4.99.1.2 organomercurial salts + H+ primary, secondary, tertiary, alkyl, vinyl, allyl, aryl Escherichia coli ?
-
 ?
4.99.1.2 phenyl mercuric acetate + H+
-
 Escherichia coli benzene + Hg2+ + acetate
-
 ?
4.99.1.2 RHg+ + H+
-
 Escherichia coli RH + Hg2+
-
 ir
4.99.1.2 sec-butyl mercuric bromide + H+
-
 Escherichia coli ?
-
 ?
4.99.1.2 tert-butyl mercuric chloride + H+
-
 Escherichia coli ?
-
 ?
4.99.1.2 vinyl mercuric bromide + H+
-
 Escherichia coli ethene + Hg+ + Br-
-
 ?

Subunits

EC Number Subunits Comment Organism
4.99.1.2 monomer 1 * 22400, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.99.1.2 37
-
 enzyme assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.99.1.2 additional information
-
 additional information
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.99.1.2 additional information no cofactors Escherichia coli