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Literature summary extracted from

  • Staudacher, E.; Dalik, T.; Wawra, P.; Altmann, F.; Maerz, L.
    Functional purification and characterization of a GDP-fucose: beta-N-acetylglucosamine (Fuc to Asn linked GlcNAc) alpha1,3-fucosyltransferase from mung beans (1995), Glycoconj. J., 12, 780-786.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.214 Triton X-100 0.1-0.5% slightly enhances activity Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.214 N-ethylmaleimide not inhibitory Vigna radiata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.214 0.06
-
 GnGnF6-peptide
-
 Vigna radiata
2.4.1.214 0.26
-
 GnGn-peptide
-
 Vigna radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.214 Mn2+ optimal activity at 10-15 mM Vigna radiata
2.4.1.214 Zn2+
-
 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.214 64000
-
 gel filtration Vigna radiata
2.4.1.214 65000
-
 1 * 65000, SDS-PAGE Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.214 Vigna radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.214 by chromatography on DE52 cellulose and Affigel Blue, chromatofocusing using PBE 94 and polybuffer 96, gelfiltration on a Sephacryl S200 column and affinity chromatography on a GnGn-column Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.214 seed
-
 Vigna radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.214 0.071
-
-
 Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.214 GDP-fucose + GnGn-oligosaccharide 93% relative activity of pure enzyme Vigna radiata GDP + alpha1-3 fucosylated GnGn-oligosaccharide
-
 ?
2.4.1.214 GDP-fucose + GnGn-peptide 100% relative activity of pure enzyme and of crude extract Vigna radiata GDP + alpha1-3 fucosylated peptide
-
 ?
2.4.1.214 GDP-fucose + GnGnF6-oligosaccharide 95% relative activity of pure enzyme Vigna radiata GDP + alpha1-3 fucosylated oligosaccharide
-
 ?
2.4.1.214 GDP-fucose + GnGnF6-peptide 99% relative activity of pure enzyme and 70% relative activity of crude extract Vigna radiata GDP + alpha1-3-fucosylated GnGnF6-peptide
-
 ?
2.4.1.214 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Vigna radiata GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
2.4.1.214 additional information the enzyme acts upon N-glycopeptides and related oligosaccharides with the glycan structure GlcNAc2Man3GlcNAc2, no transfer to N-glycans is observed when the terminal GlcNAc residues are either absent or substituted with galactose, N-acetyllactosamine, lacto-N-biose and N-acetylchito-oligosaccharides do not function as acceptors for the enzyme Vigna radiata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.214 monomer 1 * 65000, SDS-PAGE Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.214 7
-
-
 Vigna radiata