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Literature summary extracted from

  • Scudder, P.R.; Chantler, E.N.
    Glycosyltransferases of the human cervical epithelium. I. Characterization of a beta-galactoside alpha-2-L-fucosyltransferase and the identification of a beta-N-acetylglucosaminide alpha-3-L-fucosyltransferase (1981), Biochim. Biophys. Acta, 660, 128-135.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.69 GTP 0.002 mM, stimulation to 160% of the control reaction Homo sapiens
2.4.1.69 Triton X-100 required Homo sapiens

General Stability

EC Number General Stability Organism
2.4.1.69 freezing and thawing has no effect Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 GDP competitive Homo sapiens
2.4.1.69 GTP above 0.02 mM Homo sapiens
2.4.1.69 NEM 3 mM, almost complete inhibition Homo sapiens
2.4.1.69 PCMB
-
 Homo sapiens
2.4.1.214 N-ethylmaleimide at 3 mM Homo sapiens
2.4.1.214 p-chloromercuribenzoate at 0.05 mM: almost 30% of the original enzyme activity remains Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 0.032
-
 GDP-L-fucose
-
 Homo sapiens
2.4.1.69 8.2
-
 phenyl beta-D-galactoside
-
 Homo sapiens
2.4.1.69 33
-
 di-N-acetylchitobiose
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 Mg2+ enzyme requires either Mn2+ or Mg2+ for maximal activity Homo sapiens
2.4.1.69 Mn2+ enzyme requires either Mn2+ or Mg2+ for maximal activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R Homo sapiens the acid form of the enzyme is probably involved in glycoprotein synthesis in vivo GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.214 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine Homo sapiens
-
 GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Homo sapiens
-
-
-
2.4.1.214 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.69 cervical epithelium
-
 Homo sapiens
-
2.4.1.214 cervical epithelium
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.69 -20°C, stable for at least 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-L-fucose + agalacto-asialofetuin
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-agalacto-asialofetuin
-
 ?
2.4.1.69 GDP-L-fucose + asialo alpha1 acid glycoprotein
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-asialo alpha1 acid glycoprotein
-
 ?
2.4.1.69 GDP-L-fucose + asialofetuin
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-asialofetuin
-
 ?
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R the acid form of the enzyme is probably involved in glycoprotein synthesis in vivo Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.69 GDP-L-fucose + D-galactose
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-D-galactose
-
 ?
2.4.1.69 GDP-L-fucose + di-N-acetylchitobiose
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.69 GDP-L-fucose + fetuin
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-fetuin
-
 ?
2.4.1.69 GDP-L-fucose + lacto-N-biose 1 i.e. Gal(1-3)GlcNAc Homo sapiens GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
 ?
2.4.1.69 GDP-L-fucose + lactose
-
 Homo sapiens GDP + fucosyllactose
-
 ?
2.4.1.69 GDP-L-fucose + N-acetylallolactosamine
-
 Homo sapiens GDP + ?
-
 ?
2.4.1.69 GDP-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
-
 ?
2.4.1.69 GDP-L-fucose + phenyl-beta-D-galactoside
-
 Homo sapiens GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
 ?
2.4.1.214 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.69 25
-
 at pH 6.0 Homo sapiens
2.4.1.214 37
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 6
-
-
 Homo sapiens
2.4.1.214 7.2
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.69 0.05
-
 GDP
-
 Homo sapiens