Literature summary extracted from

Livesey, G.; Lund, P.
Determination of branched-chain amino and keto acids with leucine dehydrogenase (1988), Methods Enzymol., 166, 3-10.

Application

EC Number	Application	Comment	Organism
1.4.1.9	analysis	cheap and rapid determination of branched-chain amino and oxo acids	Bacillus subtilis
1.4.1.9	analysis	cheap and rapid determination of branched-chain amino and oxo acids	Lysinibacillus sphaericus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.9	Bacillus subtilis	-	-	-
1.4.1.9	Lysinibacillus sphaericus	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.1.9	L-Ile + H2O + NAD+	-	Bacillus subtilis	3-methyl-2-oxopentanoate + NH3 + NADH	-	r
1.4.1.9	L-Ile + H2O + NAD+	-	Lysinibacillus sphaericus	3-methyl-2-oxopentanoate + NH3 + NADH	-	r
1.4.1.9	L-Leu + H2O + NAD+	-	Bacillus subtilis	4-methyl-2-oxopentanoate + NH3 + NADH	-	r
1.4.1.9	L-Leu + H2O + NAD+	-	Lysinibacillus sphaericus	4-methyl-2-oxopentanoate + NH3 + NADH	-	r
1.4.1.9	L-Val + H2O + NAD+	-	Bacillus subtilis	3-methyl-2-oxobutanoate + NH3 + NADH	-	?
1.4.1.9	L-Val + H2O + NAD+	-	Lysinibacillus sphaericus	3-methyl-2-oxobutanoate + NH3 + NADH	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.1.9	NAD+	-	Bacillus subtilis
1.4.1.9	NAD+	-	Lysinibacillus sphaericus
1.4.1.9	NADH	-	Bacillus subtilis
1.4.1.9	NADH	-	Lysinibacillus sphaericus

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Release 2023.1 (January 2023)