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Literature summary extracted from

  • Ohshima, T.; Nishida, N.; Bakthavatsalam, S.; Kataoka, K.; Takada, H.; Yoshimura, T.; Esaki, N.; Soda, K.
    The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius (1994), Eur. J. Biochem., 222, 305-312.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9
-
 Thermoactinomyces intermedius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.9 0.042
-
 NADH
-
 Thermoactinomyces intermedius
1.4.1.9 0.36
-
 NAD+
-
 Thermoactinomyces intermedius
1.4.1.9 0.4
-
 L-Ile
-
 Thermoactinomyces intermedius
1.4.1.9 0.63
-
 4-methyl-2-oxopentanoate
-
 Thermoactinomyces intermedius
1.4.1.9 0.91
-
 2-oxopentanoate
-
 Thermoactinomyces intermedius
1.4.1.9 1.4
-
 2-Oxohexanoate
-
 Thermoactinomyces intermedius
1.4.1.9 2
-
 L-Leu
-
 Thermoactinomyces intermedius
1.4.1.9 2.2
-
 2-oxo-3-methylpentanoate
-
 Thermoactinomyces intermedius
1.4.1.9 2.4
-
 L-Val
-
 Thermoactinomyces intermedius
1.4.1.9 2.7
-
 4-methyl-2-oxo-5,5,5-trifluoropentanoate
-
 Thermoactinomyces intermedius
1.4.1.9 2.8
-
 NADP+ mutant enzyme D203A/I204R Thermoactinomyces intermedius
1.4.1.9 4.4
-
 3-methyl-2-oxobutanoate
-
 Thermoactinomyces intermedius
1.4.1.9 7.2
-
 2-oxobutanoate
-
 Thermoactinomyces intermedius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.9 42000
-
 4 * 42000, SDS-PAGE Thermoactinomyces intermedius
1.4.1.9 340000
-
 gel filtration Thermoactinomyces intermedius

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Thermoactinomyces intermedius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.9
-
 Thermoactinomyces intermedius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.9 102
-
-
 Thermoactinomyces intermedius
1.4.1.9 122
-
 recombinant enzyme Thermoactinomyces intermedius

Storage Stability

EC Number Storage Stability Organism
1.4.1.9 4°C, stable for over two years Thermoactinomyces intermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 2-oxohexanoate + NH3 + NADH + H+ 46% activity compared to 2-oxoisocaproate Thermoactinomyces intermedius L-norleucine + H2O + NAD+
-
 r
1.4.1.9 4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH
-
 Thermoactinomyces intermedius 2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O
-
 ?
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
 Thermoactinomyces intermedius 2-oxobutanoate + NH3 + NADH + H+
-
 ?
1.4.1.9 L-2-aminobutanoate + H2O + NAD+
-
 Thermoactinomyces intermedius 2-oxobutanoate + NH3 + NADH + H+
-
 r
1.4.1.9 L-Ile + H2O + NAD+
-
 Thermoactinomyces intermedius 3-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Leu + H2O + NAD+
-
 Thermoactinomyces intermedius 4-methyl-2-oxopentanoate + NH3 + NADH
-
 r
1.4.1.9 L-Val + H2O + NAD+
-
 Thermoactinomyces intermedius 3-methyl-2-oxobutanoate + NH3 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.9 octamer 4 * 42000, SDS-PAGE Thermoactinomyces intermedius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.9 65
-
 10 min, retains its full activity Thermoactinomyces intermedius
1.4.1.9 70
-
 40 min, 3 M NaCl, less than 10% loss of activity Thermoactinomyces intermedius
1.4.1.9 75
-
 10 min, about 75% loss of activity Thermoactinomyces intermedius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 9
-
 reductive amination of 3-methyl-2-oxobutanoate Thermoactinomyces intermedius
1.4.1.9 9.3
-
 reductive amination of 2-oxo-3-methylpentanoate Thermoactinomyces intermedius
1.4.1.9 9.6
-
 reductive amination of 4-methyl-2-oxopentanoate Thermoactinomyces intermedius
1.4.1.9 10
-
 deamination of L-Val Thermoactinomyces intermedius
1.4.1.9 10.5
-
 deamination of L-Leu Thermoactinomyces intermedius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.9 6 10.9 50°C, 5 min, stable Thermoactinomyces intermedius
1.4.1.9 7.5
-
 70°C, 5 min, most stable at pH 7.5 Thermoactinomyces intermedius

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NAD+
-
 Thermoactinomyces intermedius
1.4.1.9 NADH
-
 Thermoactinomyces intermedius