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Literature summary extracted from

  • Asano, Y.; Tanetani, M.
    Thermostable phenylalanine dehydrogenase from a mesophilic Microbacterium sp. strain DM 86-1 (1998), Arch. Microbiol., 169, 220-224.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.20 HgCl2 1.0 mM, 97% inhibition Microbacterium sp.
1.4.1.20 PCMB 1.0 mM, 98% loss of activity Microbacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.07
-
 NADH
-
 Microbacterium sp.
1.4.1.20 0.1
-
 L-Phe
-
 Microbacterium sp.
1.4.1.20 0.2
-
 NAD+
-
 Microbacterium sp.
1.4.1.20 0.3
-
 phenylpyruvate
-
 Microbacterium sp.
1.4.1.20 1.54
-
 L-norleucine
-
 Microbacterium sp.
1.4.1.20 1.6
-
 L-Met
-
 Microbacterium sp.
1.4.1.20 85
-
 NH3
-
 Microbacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 41000
-
 8 * 41000, SDS-PAGE Microbacterium sp.
1.4.1.20 330000
-
 gel filtration Microbacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Microbacterium sp.
-
-
-
1.4.1.20 Microbacterium sp. DM 86-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20
-
 Microbacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.20 37.1
-
-
 Microbacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 L-Leu + H2O + NAD+
-
 Microbacterium sp. 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
 ?
1.4.1.20 L-Met + H2O + NAD+
-
 Microbacterium sp. 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
 ?
1.4.1.20 L-norleucine + H2O + NAD+
-
 Microbacterium sp. 2-oxohexanoic acid + NADH + NH3
-
 ?
1.4.1.20 L-norleucine + H2O + NAD+
-
 Microbacterium sp. DM 86-1 2-oxohexanoic acid + NADH + NH3
-
 ?
1.4.1.20 L-norvaline + H2O + NAD+
-
 Microbacterium sp. 2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.20 L-norvaline + H2O + NAD+
-
 Microbacterium sp. DM 86-1 2-oxopentanoate + NH3 + NADH
-
 ?
1.4.1.20 L-Phe + H2O + NAD+
-
 Microbacterium sp. phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-Phe + H2O + NAD+
-
 Microbacterium sp. DM 86-1 phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-Tyr + H2O + NAD+
-
 Microbacterium sp. 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?
1.4.1.20 L-Tyr + H2O + NAD+
-
 Microbacterium sp. DM 86-1 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?
1.4.1.20 L-Val + H2O + NAD+
-
 Microbacterium sp. 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?
1.4.1.20 L-Val + H2O + NAD+
-
 Microbacterium sp. DM 86-1 3-methyl-2-oxobutanoate + NH3 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.20 octamer 8 * 41000, SDS-PAGE Microbacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.20 70
-
-
 Microbacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.20 55
-
 10 min, stable Microbacterium sp.
1.4.1.20 60
-
 pH 7.0, 10 min, stable Microbacterium sp.
1.4.1.20 65 70 pH 7.0, 10 min, 50% loss of activity Microbacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 12
-
 oxidative deamination and reductive amination Microbacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.20 8 12.5 30°C, 30 min, stable Microbacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
 Microbacterium sp.
1.4.1.20 NADH
-
 Microbacterium sp.