Literature summary extracted from

Vadas, A.J.; Schroder, I.; Monbouquette, H.G.
Room-temperature synthesis of L-alanine using the alanine dehydrogenase of the hyperthermophilic archaeon archaeoglobusfulgidus (2002), Biotechnol. Prog., 18, 909-911.

Application

EC Number	Application	Comment	Organism
1.4.1.1	synthesis	batch synthesis of L-Ala at room temperature via reductive amination of pyruvate	Archaeoglobus fulgidus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.4.1.1	Archaeoglobus fulgidus	-	glycine and alanine dehydrogenase activities are catalyzed by the same proteon in Mycobacterium smegamatis	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.4.1.1	-	Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.4.1.1	60	-	25°C	Archaeoglobus fulgidus
1.4.1.1	203	-	82°C	Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.4.1.1	L-Ala + H2O + NAD+	-	Archaeoglobus fulgidus	pyruvate + NH3 + NADH	-	r

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.4.1.1	22	-	139 h, no loss of activity	Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.4.1.1	additional information	-	additional information	-	Archaeoglobus fulgidus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.4.1.1	NAD+	-	Archaeoglobus fulgidus
1.4.1.1	NADH	-	Archaeoglobus fulgidus

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Release 2023.1 (January 2023)