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Literature summary extracted from

  • Smith, M.T.; Emerich, D.W.
    Alanine dehydrogenase from soybean nodule bacteroids: purification and properties (1993), Arch. Biochem. Biophys., 304, 379-385.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 L-Ala substrate inhibition Rhizobium sp.
1.4.1.1 methylglyoxal
-
 Rhizobium sp.
1.4.1.1 NADH product inhibition Rhizobium sp.
1.4.1.1 NH4+
-
 Rhizobium sp.
1.4.1.1 oxamate
-
 Rhizobium sp.
1.4.1.1 propionate
-
 Rhizobium sp.
1.4.1.1 pyruvate substrate inhibition Rhizobium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.022
-
 NADH
-
 Rhizobium sp.
1.4.1.1 0.098
-
 NADH
-
 Rhizobium sp.
1.4.1.1 0.37
-
 L-Ala
-
 Rhizobium sp.
1.4.1.1 0.43
-
 pyruvate
-
 Rhizobium sp.
1.4.1.1 5.5
-
 NH4+
-
 Rhizobium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Rhizobium sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.1 L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH + H+ the kinetic mechanism at pH 8.5 is determined to be ter-bi Theorell-Chance. In the amination direction, the substrates add in the order: NADH, NH4+, pyruvate, with NH4+ binding in rapid-equilibrium. In the reverse direction, NAD adds first followed by L-Ala Rhizobium sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Rhizobium sp. pyruvate + NH3 + NADH
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Rhizobium sp.
1.4.1.1 NADH
-
 Rhizobium sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.1 0.092
-
 pyruvate
-
 Rhizobium sp.
1.4.1.1 1
-
 L-Ala
-
 Rhizobium sp.
1.4.1.1 3.7
-
 NH4+
-
 Rhizobium sp.