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Literature summary extracted from
- Partial purification and properties of Halobacterium cutirubrum L-alanine dehydrogenase (1977), Biochem. J., 161, 313-320.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.4.1.1 | absolute requirement of high ionic strength for stability | Halobacterium salinarum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | Cd2+ | 10 mM, 36% inhibition of reductive amination | Halobacterium salinarum |  |
| 1.4.1.1 | Co2+ | 10 mM, 60% inhibition of reductive amination | Halobacterium salinarum | |
| 1.4.1.1 | Cu2+ | 10 mM, 60% inhibition of reductive amination | Halobacterium salinarum | |
| 1.4.1.1 | D-2-aminobutanoate | - |
Halobacterium salinarum | |
| 1.4.1.1 | D-Ala | - |
Halobacterium salinarum | |
| 1.4.1.1 | Fe2+ | 10 mM, 85% inhibition of reductive amination | Halobacterium salinarum | |
| 1.4.1.1 | Fe3+ | 10 mM, 85% inhibition of reductive amination | Halobacterium salinarum | |
| 1.4.1.1 | Mn2+ | 10 mM, 60% inhibition of reductive amination | Halobacterium salinarum | |
| 1.4.1.1 | Ni2+ | 10 mM, 60% inhibition of reductive amination | Halobacterium salinarum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.1 | 0.2 | - |
NADH | - |
Halobacterium salinarum | |
| 1.4.1.1 | 0.5 | - |
NAD+ | - |
Halobacterium salinarum | |
| 1.4.1.1 | 0.8 | - |
pyruvate | - |
Halobacterium salinarum | |
| 1.4.1.1 | 7 | - |
L-Ala | - |
Halobacterium salinarum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | Cs+ | reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+ | Halobacterium salinarum | |
| 1.4.1.1 | KCl | reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+, absolute requirement for K+ in oxidative deamination, completely inactive with Na+ or NH4+ | Halobacterium salinarum | |
| 1.4.1.1 | Li+ | reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+ | Halobacterium salinarum | |
| 1.4.1.1 | additional information | absolute requirement for high ionic strength for optimum activity | Halobacterium salinarum | |
| 1.4.1.1 | NaCl | reductive amination is activated equally well by K+, Na+ or NH4+ and to a lesser extent with Cs+ or Li+ | Halobacterium salinarum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 72500 | - |
- |
Halobacterium salinarum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.1 | Halobacterium salinarum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.1 | partial | Halobacterium salinarum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 168 | - |
- |
Halobacterium salinarum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.1 | 2-oxoglutarate + NH3 + NADH | no activity | Halobacterium salinarum | L-Gln + NAD+ + H2O | - |
? | |
| 1.4.1.1 | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
Halobacterium salinarum | 2-amino-4-methylpentanoate + NAD+ + H2O | - |
? | |
| 1.4.1.1 | glyoxylate + NH3 + NADH | - |
Halobacterium salinarum | aminoacetate + NAD+ + H2O | - |
? | |
| 1.4.1.1 | L-2-aminobutanoate + H2O + NAD+ | - |
Halobacterium salinarum | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Halobacterium salinarum | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | oxaloacetate + NH3 + NADH | - |
Halobacterium salinarum | L-Asp + H2O + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.1.1 | hexamer | - |
Halobacterium salinarum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 60 | 65 | reductive amination, in presence of KCl | Halobacterium salinarum |
| 1.4.1.1 | 65 | 70 | reductive amination in presence of NaCl | Halobacterium salinarum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 40 | 65 | 40°C: about 50% of activity maximum, 65°C: optimum, sharp decrease of activity above, no activity at 75°C | Halobacterium salinarum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 55 | - |
pH 8.5, 50 min, 40% loss of activity | Halobacterium salinarum |
| 1.4.1.1 | 60 | - |
pH 8.5, 10 min, about 45% loss of activity | Halobacterium salinarum |
| 1.4.1.1 | 70 | - |
pH 8.5, 10 min, 90% loss of activity | Halobacterium salinarum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 9 | - |
both directions | Halobacterium salinarum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | NAD+ | - |
Halobacterium salinarum | |
| 1.4.1.1 | NADH | - |
Halobacterium salinarum | |
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