EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.1.1 | - |
Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.1 | D-Ala | - |
Bacillus subtilis | |
1.4.1.1 | D-Ala | - |
Thermus thermophilus | |
1.4.1.1 | D-Cys | - |
Bacillus subtilis | |
1.4.1.1 | D-Cys | - |
Thermus thermophilus | |
1.4.1.1 | L-Cys | - |
Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.1 | 0.023 | - |
NADH | - |
Bacillus subtilis | |
1.4.1.1 | 0.035 | - |
NADH | - |
Bacillus subtilis | |
1.4.1.1 | 0.035 | - |
NADH | - |
Thermus thermophilus | |
1.4.1.1 | 0.12 | - |
NAD+ | - |
Thermus thermophilus | |
1.4.1.1 | 0.18 | - |
NAD+ | - |
Bacillus subtilis | |
1.4.1.1 | 0.54 | - |
pyruvate | - |
Bacillus subtilis | |
1.4.1.1 | 0.75 | - |
pyruvate | - |
Thermus thermophilus | |
1.4.1.1 | 1.7 | - |
L-Ala | - |
Bacillus subtilis | |
1.4.1.1 | 4.2 | - |
L-Ala | - |
Thermus thermophilus | |
1.4.1.1 | 38 | - |
NH4+ | - |
Bacillus subtilis | |
1.4.1.1 | 59 | - |
NH3 | - |
Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.1 | 48000 | - |
6 * 48000, SDS-PAGE | Thermus thermophilus |
1.4.1.1 | 290000 | - |
equilibrium sedimentation | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.1 | Bacillus subtilis | - |
- |
- |
1.4.1.1 | Thermus thermophilus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.1 | - |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.1 | L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
r | |
1.4.1.1 | L-Ala + H2O + NAD+ | - |
Thermus thermophilus | pyruvate + NH3 + NADH | - |
r | |
1.4.1.1 | L-Ile + H2O + NAD+ | 0.1% of the activity with L-Ala | Thermus thermophilus | 2-oxo-3-methylpentanoate + NH3 + NADH | - |
? | |
1.4.1.1 | L-Ile + H2O + NAD+ | 5% of the activity with L-Ala | Bacillus subtilis | 2-oxo-3-methylpentanoate + NH3 + NADH | - |
? | |
1.4.1.1 | L-Ser + H2O + NAD+ | - |
Bacillus subtilis | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
1.4.1.1 | L-Ser + H2O + NAD+ | - |
Thermus thermophilus | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
1.4.1.1 | L-Val + H2O + NAD+ | 9% of the activity with L-Ala | Bacillus subtilis | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.1 | L-Val + H2O + NAD+ | 0.2% of the activity with L-Ala | Thermus thermophilus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.1 | hexamer | - |
Bacillus subtilis |
1.4.1.1 | hexamer | 6 * 48000, SDS-PAGE | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.1 | 63 | - |
melting temperature | Bacillus subtilis |
1.4.1.1 | 86 | - |
melting temperature | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.1 | 8 | - |
- |
Thermus thermophilus |
1.4.1.1 | 8.5 | - |
- |
Thermus thermophilus |
1.4.1.1 | 8.5 | - |
reductive amination of pyruvate | Bacillus subtilis |
1.4.1.1 | 10.5 | - |
deamination of L-Ala | Thermus thermophilus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.1 | NAD+ | - |
Bacillus subtilis | |
1.4.1.1 | NAD+ | - |
Thermus thermophilus | |
1.4.1.1 | NADH | - |
Bacillus subtilis | |
1.4.1.1 | NADH | - |
Thermus thermophilus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.1 | 0.64 | - |
D-Cys | - |
Bacillus subtilis | |
1.4.1.1 | 0.9 | - |
D-Cys | - |
Bacillus subtilis | |
1.4.1.1 | 5 | - |
D-Ala | - |
Bacillus subtilis | |
1.4.1.1 | 20 | - |
D-Ala | - |
Bacillus subtilis |