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Literature summary extracted from

  • El-Awamry, Z.A.; El-Rahmany, T.A.
    Partial purification and properties of Cunninghamella elegans L-alanine dehydrogenase (1989), Zentralbl. Mikrobiol., 144, 231-240.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.1 dialysis against 0.02 M Tris-HCl, pH 8.4, 75% loss of activity after 3 h, complete loss of activity after 24 h Cunninghamella elegans
1.4.1.1 freezing and thawing completely destroys activity Cunninghamella elegans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 EDTA
-
 Cunninghamella elegans
1.4.1.1 iodoacetate
-
 Cunninghamella elegans
1.4.1.1 Zn2+
-
 Cunninghamella elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.1 Ca2+ activation Cunninghamella elegans
1.4.1.1 Co2+ activation Cunninghamella elegans
1.4.1.1 Fe2+ activation Cunninghamella elegans
1.4.1.1 Mg2+ activation Cunninghamella elegans
1.4.1.1 Mn2+ activation Cunninghamella elegans

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Cunninghamella elegans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 partial Cunninghamella elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.1 mycelium
-
 Cunninghamella elegans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 additional information
-
-
 Cunninghamella elegans

Storage Stability

EC Number Storage Stability Organism
1.4.1.1 4°C, 0.02 M Tris-HCl buffer, pH 8.4, stable for 1 d, then the activity decreases gradually till the sixth day at which complete loss of activity occurs Cunninghamella elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+ absolutely specific for L-Ala in oxidative deamination Cunninghamella elegans pyruvate + NH3 + NADH
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 40
-
-
 Cunninghamella elegans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 50
-
 35% loss of activity after 10 min, 65% loss of activity after 20 min Cunninghamella elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8
-
 reductive amination Cunninghamella elegans
1.4.1.1 10
-
 oxidative deamination Cunninghamella elegans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.1 6.5 9 pH 6.5: about 30% of activity maximum, pH 9.0: about 55% of maximal activity, reductive amination Cunninghamella elegans
1.4.1.1 8 10.5 pH 8.0: about 60% of activity maximum, pH 10.5: about 80% of activity maximum, oxidative deamination Cunninghamella elegans

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Cunninghamella elegans
1.4.1.1 NADH
-
 Cunninghamella elegans