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Literature summary extracted from
- Purification and properties of trehalose-synthesizing enzyme from Pseudomonas sp. F1 (1997), J. Ferment. Bioeng., 84, 358-360.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.16 | 1.1 | - |
maltose | - |
Pseudomonas sp. |  |
| 5.4.99.16 | 26 | - |
trehalose | - |
Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 67000 | - |
4 * 67000, SDS-PAGE | Pseudomonas sp. |
| 5.4.99.16 | 250000 | - |
gel filtration | Pseudomonas sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.16 | Pseudomonas sp. | - |
- |
- |
| 5.4.99.16 | Pseudomonas sp. F1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.16 | - |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 41.2 | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.16 | maltose | - |
Pseudomonas sp. | alpha,alpha-trehalose | - |
? | |
| 5.4.99.16 | maltose | - |
Pseudomonas sp. F1 | alpha,alpha-trehalose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.16 | tetramer | 4 * 67000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 45 | - |
- |
Pseudomonas sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 55 | - |
pH 7.0, 1 h, stable below | Pseudomonas sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 8 | 9 | - |
Pseudomonas sp. |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.16 | 7 | 9 | 37°C, 1 h, stable | Pseudomonas sp. |
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