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Literature summary extracted from

  • Podschun, B.; Jahnke, K.; Schnackerz, K.D.; Cook, P.F.
    Acid base catalytic mechanism of the dihydropyrimidine dehydrogenase from pH studies (1993), J. Biol. Chem., 268, 3407-3413.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.2 medicine target for inhibitor design to enhance the cytotoxical effect of 5-fluorouracil in tumor cells by inhibiting the DPD activity with 5-fluorouracil as substrate Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.2 5,6-dihydrouracil competitive Sus scrofa
1.3.1.2 ATP-ribose competitive Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.2 cytoplasm
-
 Sus scrofa 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2
-
 Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.2 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ mechanism, rate-determining half-reaction for reduction of flavin by NADPH has minor rate limitation, while the following protonation of flavin at N-1 is slow Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 0.417 0.5 purified enzyme Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 thymine + NADPH
-
 Sus scrofa dihydrothymine + NADP+
-
 ?
1.3.1.2 uracil + NADPH
-
 Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.2 additional information
-
 investigation of pH-dependency of the reaction and inhibition by 2,6-dihydrouracil and ATP-ribose, kinetics Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.2 5.8 8.5
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 flavin
-
 Sus scrofa
1.3.1.2 NADPH strictly dependent on Sus scrofa