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Literature summary extracted from

  • Schmitt, U.; Jahnke, K.; Rosenbaum, K.; Cook, P.F.; Schnackerz, K.D.
    Purification and characterization of dihydropyrimidine dehydrogenase from Alcaligenes eutrophus (1996), Arch. Biochem. Biophys., 332, 175-182.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.2 Sulfide 23.4 mol per mol of enzyme Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.2 3-aminopyridine ADP
-
 Cupriavidus necator
1.3.1.2 5,6-dihydrouracil
-
 Cupriavidus necator
1.3.1.2 ATP-ribose dead-end inhibition Cupriavidus necator
1.3.1.2 additional information not: thio-NADPH, cyclo-NADPH, deamino-NADPH Cupriavidus necator
1.3.1.2 NADP+ competitive versus NADPH Cupriavidus necator
1.3.1.2 NADPH
-
 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.2 0.0049
-
 Uracil
-
 Cupriavidus necator
1.3.1.2 0.0068
-
 NADPH in presence of uracil Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.2 Iron iron atoms are probably present in iron-sulfur centers Cupriavidus necator
1.3.1.2 Iron 24.5 mol iron per mol of enzyme Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 52000
-
 4 * 52000, SDS-PAGE Cupriavidus necator
1.3.1.2 207000 210000 gel fitration or gradient native PAGE Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.2 uracil + NADPH Cupriavidus necator rate limiting step in catabolic breakdown of uracil to beta-alanine 5,6-dihydrouracil + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2
-
 Cupriavidus necator

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.2 5,6-dihydrouracil + NADP+ = uracil + NADPH + H+ abstraction of pro-S hydrogen of NADPH and thus B-side stereospecific class dehydrogenase Cupriavidus necator
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 182.1
-
 purified enzyme Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.3.1.2 -80°C, 35 mM potassium phosphate, pH 7.4, 1 mM dithioerythritol, more than 2 months without loss of activity, without dithioerythritol 50% loss of activity Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 5-aminouracil + NADPH
-
 Cupriavidus necator ?
-
 ?
1.3.1.2 5-fluorouracil + NADPH + H+
-
 Cupriavidus necator 5-fluoro-5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 dihydrothymine + NADP+
-
 Cupriavidus necator thymine + NADPH
-
 r
1.3.1.2 additional information not: orotate, cytosine Cupriavidus necator ?
-
 ?
1.3.1.2 uracil + NADPH
-
 Cupriavidus necator 5,6-dihydrouracil + NADP+
-
 ?
1.3.1.2 uracil + NADPH rate limiting step in catabolic breakdown of uracil to beta-alanine Cupriavidus necator 5,6-dihydrouracil + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.2 tetramer 4 * 52000, SDS-PAGE Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.2 30
-
 assay at Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 flavin 4.1 mol FMN per mole of enzyme Cupriavidus necator
1.3.1.2 NADP+
-
 Cupriavidus necator
1.3.1.2 NADPH
-
 Cupriavidus necator

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.2 additional information
-
 additional information Ki for ATP-ribose, 3-aminopyridine ADP, and 2,6-dihydrouracil Cupriavidus necator
1.3.1.2 additional information
-
 additional information inhibitory reactions between uracil, NADP+ and dihydrouracil Cupriavidus necator
1.3.1.2 0.007
-
 NADPH
-
 Cupriavidus necator
1.3.1.2 0.011
-
 NADP+
-
 Cupriavidus necator
1.3.1.2 0.15
-
 dihydrouracil
-
 Cupriavidus necator