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Literature summary extracted from

  • Podschun, B.; Wahler, G.; Schnackerz, K.D.
    Purification and characterization of dihydropyrimidine dehydrogenase from pig liver (1989), Eur. J. Biochem., 185, 219-224.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.2 medicine rate limiting enzyme in catabolic degradation of pyrimidine derivatives, selective inhibition is strategy for design of antitumor, antimicrobial and potentially antiparasitic agents Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.2 cytoplasm
-
 Sus scrofa 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.2 Iron iron atoms are probably present in iron-sulfur centers Sus scrofa
1.3.1.2 Iron contains 32 iron atoms per 206000 MW protein Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.2 107000
-
 2 * 107000, SDS-PAGE Sus scrofa
1.3.1.2 206000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.2
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.2 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.2 0.35
-
-
 Sus scrofa
1.3.1.2 21
-
 purified enzyme Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.3.1.2 -20°C, protein concentration 0.5-1 mg/ml, 1 mM dithiothreitol, stable for several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.2 5-aminouracil + NADPH
-
 Sus scrofa ?
-
 ?
1.3.1.2 uracil + NADPH
-
 Sus scrofa 5,6-dihydrouracil + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.2 dimer 2 * 107000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.2 37
-
 assay at Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.2 flavin contains 2 mol FMN and 2 mol FAD per mol of enzyme, tightly associated Sus scrofa
1.3.1.2 NADPH
-
 Sus scrofa