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Literature summary extracted from

  • Hester, K.; Luo, J.; Burns, B.; Braswell, E.H.; Sokatch, J.R.
    Purification of active E1alpha2beta2 of Pseudomonas putida branched-chain-oxoacid dehydrogenase (1995), Eur. J. Biochem., 233, 828-836.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.4.4 thiamine diphosphate
-
 Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.4 expression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.2.4.4 S313A twofold increase in Km-value but no change in turnover-number Pseudomonas putida
1.2.4.4 S315A mutation has no effect on Km-value or turnover-number Pseudomonas putida
1.2.4.4 S319A mutation has no effect on Km-value or turnover-number Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.4 172000
-
 equilibrium sedimentation Pseudomonas putida
1.2.4.4 190000
-
 E1 component, gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.4 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.4
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.4.4 26
-
-
 Pseudomonas putida
1.2.4.4 38
-
 E1 component expressed in Escherichia coli Pseudomonas putida

Subunits

EC Number Subunits Comment Organism
1.2.4.4 tetramer alpha2,beta2, MW of alpha is 45000 Da, expression of bkdA2 yields the 39000 Da form and the 37000 Da form of the beta-subunit, both forms of the E1 beta-subunit are produced in Pseudomonas putida, only the 39000 Da protein is produced in Escherichia coli, SDS-PAGE Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.4 CoA
-
 Pseudomonas putida
1.2.4.4 NAD+
-
 Pseudomonas putida