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Literature summary extracted from

  • Hirashima, M.; Hayakawa, T.; Koike, M.
    Mammalian alpha-keto acid dehydrogenase complexes. II. An improved procedure for the preparation of 2-oxoglutarate dehydrogenase complex from pig heart muscle (1967), J. Biol. Chem., 242, 902-907.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.105 Ca2+ activates Sus scrofa
1.2.1.105 Mg2+ activates 2-oxoglutarate dehydrogenase complex Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.105 additional information
-
 2700000 is the MW of the 2-oxoglutarate dehydrogenase complex, gel filtration Sus scrofa
1.2.1.105 additional information
-
 2800000 Da is the MW of the 2-oxoglutarate dehydrogenase complex, calculation from sedimentation and diffusion data Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.105 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.105 2-oxoglutarate dehydrogenase complex Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.105 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.105 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.105 2-oxoglutarate + lipoamide
-
 Sus scrofa S-succinyldihydrolipoamide + CO2
-
 ?
1.2.1.105 additional information enzyme complex is also active with 2-oxoadipate Sus scrofa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.105 30 35 2-oxoglutarate dehydrogenase activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.105 6.5
-
 2-oxoglutarate dehydrogenase reaction Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.105 additional information 2-oxoglutarate dehydrogenase complex contains protein-bound lipoic acid Sus scrofa
1.2.1.105 additional information 2-oxoglutarate dehydrogenase complex contains FAD Sus scrofa
1.2.1.105 thiamine diphosphate 2-oxoglutarate dehydrogenase complex contains thiamine diphosphate Sus scrofa