BRENDA - Enzyme Database show

alpha-Ketoglutarate dehydrogenase from pig heart

Sanadi, D.R.; Methods Enzymol. 13, 52-55 (1969)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.4.2
arsenite
probably bind the dithiol group in the lipoic acid
Sus scrofa
1.2.4.2
Cd2+
probably bind the dithiol group in the lipoic acid
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.4.2
0.013
-
2-oxoglutarate
-
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.4.2
mitochondrion
-
Sus scrofa
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.4.2
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.4.2
2-oxoglutarate dehydrogenase complex
Sus scrofa
Reaction
EC Number
Reaction
Commentary
Organism
1.2.4.2
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.4.2
heart
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.4.2
2-oxoglutarate + lipoamide
-
349005
Sus scrofa
S-succinyldihydrolipoamide + CO2
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.4.2
arsenite
probably bind the dithiol group in the lipoic acid
Sus scrofa
1.2.4.2
Cd2+
probably bind the dithiol group in the lipoic acid
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.4.2
0.013
-
2-oxoglutarate
-
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.4.2
mitochondrion
-
Sus scrofa
5739
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.4.2
2-oxoglutarate dehydrogenase complex
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.4.2
heart
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.4.2
2-oxoglutarate + lipoamide
-
349005
Sus scrofa
S-succinyldihydrolipoamide + CO2
-
-
-
?