Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Witzmann, S.; Bisswanger, H.
    The pyruvate dehydrogenase complex from thermophilic organisms: thermal stability and re-association from the enzyme components (1998), Biochim. Biophys. Acta, 1385, 341-352.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.104 GTP 1-10 mM, slight activation [Bacillus] caldolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 GTP 5 mM, about 50% inhibition after 3 h Geobacillus stearothermophilus
1.2.1.104 MgATP2- 5 mM, about 70% inhibition after 3 h Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.104 36000
-
 alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence Geobacillus stearothermophilus
1.2.1.104 42000
-
 alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence Geobacillus stearothermophilus
1.2.1.104 100000
-
 x * 100000, SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Geobacillus stearothermophilus
-
-
-
1.2.1.104 Thermus thermophilus
-
-
-
1.2.1.104 [Bacillus] caldolyticus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.104 additional information not regulated by phosphorylation/dephosphorylation Geobacillus stearothermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104
-
 Geobacillus stearothermophilus
1.2.1.104 pyruvate dehydrogenase can be resolved from complex by incubation with 8 M urea, 4 M guanidinium chloride, 100 mM glycine/NaOH, pH 9.0 followed by gel filtration Thermus thermophilus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.2.1.104 no reconstitution of whole complex, pyruvate dehydrogenase subunit regains some activity after separation from complex in the presence of chaperonins and ATP/MgCl2 Geobacillus stearothermophilus

Subunits

EC Number Subunits Comment Organism
1.2.1.104 ? x * 100000, SDS-PAGE Thermus thermophilus
1.2.1.104 tetramer alpha2,beta2, 2 * 42000 + 2 * 36000, SDS-PAGE, calculated from gene sequence Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.104 60
-
 pyruvate dehydrogenase complex, no loss of activity after 50 min Geobacillus stearothermophilus
1.2.1.104 72
-
 pyruvate dehydrogenase, complete loss of activity Geobacillus stearothermophilus
1.2.1.104 84
-
 pyruvate dehydrogenase [Bacillus] caldolyticus