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Literature summary extracted from

  • Reed, L.J.; Willms, C.R.
    Purification and resolution of the pyruvate dehydrogenase complex (Escherichia coli) (1966), Methods Enzymol., 9, 247-265.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 arsenite in presence of NADH, inhibition is reversed by dithiols and less effectively by monothiols Escherichia coli
1.8.1.4 Cd2+ in presence of NADH, inhibition is reversed by dithiols and less effectively by monothiols Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.1 182000 183000 calculation from sedimentation and diffusion data Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.1 pyruvate + lipoamide Escherichia coli in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide Escherichia coli additional reactions of complex, e. g. reduction of K3Fe(CN)6 S-acetyldihydrolipoamide + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Escherichia coli
-
-
-
1.8.1.4 Escherichia coli
-
 diyhdrolipoamide dehydrogenase component of the pyruvate dehydrogenase complex
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.1
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.2.4.1 -20°C, 50 mM phosphate buffer, pH 7.0, several months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.1 alpha-ketobutyrate + Fe(CN)63- + H2O
-
 Escherichia coli hydroxyacetate + CO2 + Fe(CN)64-
-
 ?
1.2.4.1 pyruvate + Fe(CN)63- + H2O
-
 Escherichia coli CO2 + Fe(CN)64-
-
 ?
1.2.4.1 pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate Escherichia coli S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide additional reactions of complex, e. g. reduction of K3Fe(CN)6 Escherichia coli S-acetyldihydrolipoamide + CO2
-
 ?
1.8.1.4 dihydrolipoamide + NAD+
-
 Escherichia coli lipoamide + NADH
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.1 thiamine diphosphate
-
 Escherichia coli
1.8.1.4 FAD 2 molecules of FAD per molecule of enzyme Escherichia coli
1.8.1.4 NAD+
-
 Escherichia coli