BRENDA - Enzyme Database show

Purification and resolution of the pyruvate dehydrogenase complex (Escherichia coli)

Reed, L.J.; Willms, C.R.; Methods Enzymol. 9, 247-265 (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.4
arsenite
in presence of NADH, inhibition is reversed by dithiols and less effectively by monothiols
Escherichia coli
1.8.1.4
Cd2+
in presence of NADH, inhibition is reversed by dithiols and less effectively by monothiols
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.4.1
182000
183000
calculation from sedimentation and diffusion data
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.4.1
pyruvate + lipoamide
Escherichia coli
in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate
S-acetyldihydrolipoamide + CO2
-
Escherichia coli
?
1.2.4.1
pyruvate + lipoamide
Escherichia coli
additional reactions of complex, e. g. reduction of K3Fe(CN)6
S-acetyldihydrolipoamide + CO2
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.4.1
Escherichia coli
-
-
-
1.8.1.4
Escherichia coli
-
diyhdrolipoamide dehydrogenase component of the pyruvate dehydrogenase complex
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.4.1
-
Escherichia coli
Storage Stability
EC Number
Storage Stability
Organism
1.2.4.1
-20C, 50 mM phosphate buffer, pH 7.0, several months, no loss of activity
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.4.1
alpha-ketobutyrate + Fe(CN)63- + H2O
-
348981
Escherichia coli
hydroxyacetate + CO2 + Fe(CN)64-
-
348981
Escherichia coli
?
1.2.4.1
pyruvate + Fe(CN)63- + H2O
-
348981
Escherichia coli
CO2 + Fe(CN)64-
-
348981
Escherichia coli
?
1.2.4.1
pyruvate + lipoamide
in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate
348981
Escherichia coli
S-acetyldihydrolipoamide + CO2
-
348981
Escherichia coli
?
1.2.4.1
pyruvate + lipoamide
additional reactions of complex, e. g. reduction of K3Fe(CN)6
348981
Escherichia coli
S-acetyldihydrolipoamide + CO2
-
348981
Escherichia coli
?
1.8.1.4
dihydrolipoamide + NAD+
-
348981
Escherichia coli
lipoamide + NADH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.4.1
thiamine diphosphate
-
Escherichia coli
1.8.1.4
FAD
2 molecules of FAD per molecule of enzyme
Escherichia coli
1.8.1.4
NAD+
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.4.1
thiamine diphosphate
-
Escherichia coli
1.8.1.4
FAD
2 molecules of FAD per molecule of enzyme
Escherichia coli
1.8.1.4
NAD+
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.4
arsenite
in presence of NADH, inhibition is reversed by dithiols and less effectively by monothiols
Escherichia coli
1.8.1.4
Cd2+
in presence of NADH, inhibition is reversed by dithiols and less effectively by monothiols
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.4.1
182000
183000
calculation from sedimentation and diffusion data
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.4.1
pyruvate + lipoamide
Escherichia coli
in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate
S-acetyldihydrolipoamide + CO2
-
Escherichia coli
?
1.2.4.1
pyruvate + lipoamide
Escherichia coli
additional reactions of complex, e. g. reduction of K3Fe(CN)6
S-acetyldihydrolipoamide + CO2
-
Escherichia coli
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.4.1
-
Escherichia coli
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.4.1
-20C, 50 mM phosphate buffer, pH 7.0, several months, no loss of activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.4.1
alpha-ketobutyrate + Fe(CN)63- + H2O
-
348981
Escherichia coli
hydroxyacetate + CO2 + Fe(CN)64-
-
348981
Escherichia coli
?
1.2.4.1
pyruvate + Fe(CN)63- + H2O
-
348981
Escherichia coli
CO2 + Fe(CN)64-
-
348981
Escherichia coli
?
1.2.4.1
pyruvate + lipoamide
in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate
348981
Escherichia coli
S-acetyldihydrolipoamide + CO2
-
348981
Escherichia coli
?
1.2.4.1
pyruvate + lipoamide
additional reactions of complex, e. g. reduction of K3Fe(CN)6
348981
Escherichia coli
S-acetyldihydrolipoamide + CO2
-
348981
Escherichia coli
?
1.8.1.4
dihydrolipoamide + NAD+
-
348981
Escherichia coli
lipoamide + NADH
-
-
-
?