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Literature summary extracted from

  • Seckler, R.; Binder, R.; Bisswanger, H.
    Purification and properties of the pyruvate dehydrogenase complex from Salmonella typhimurium and formation of hybrids with the enzyme complex from Escherichia coli (1982), Biochim. Biophys. Acta, 705, 210-217.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.104 100000
-
 2 * 100000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.4 56000
-
 x * 56000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.12 87000
-
 SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Salmonella enterica subsp. enterica serovar Typhimurium
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
1.8.1.4 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.3.1.12 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.4 from pyruvate dehydrogenase complex Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.12 E2-E3 subcomplex of pyruvate dehydrogenase, i.e. dihydrolipoamide acetyltransferase and dehydrogenase Salmonella enterica subsp. enterica serovar Typhimurium

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.2.1.104 reconstitution of pyruvate dehydrogenase complex Salmonella enterica subsp. enterica serovar Typhimurium
2.3.1.12 reconstitution of pyruvate dehydrogenase complex with components from Escherichia coli and Salmonella typhimurium Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium lipoamide + NADH
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-acetyldihydrolipoamide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.104 dimer 2 * 100000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.4 ? x * 56000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.104 thiamine diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.4 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium