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Literature summary extracted from

  • De Kok, A.; Westphal, A.H.
    Hybrid pyruvate dehydrogenase complexes reconstituted from components of the complexes from Escherichia coli and Azotobacter vinelandii (1985), Eur. J. Biochem., 152, 35-41.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA Escherichia coli
-
 S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA Azotobacter vinelandii
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Azotobacter vinelandii
-
-
-
1.2.1.104 Escherichia coli
-
-
-
2.3.1.12 Azotobacter vinelandii
-
-
-
2.3.1.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104
-
 Escherichia coli
1.2.1.104
-
 Azotobacter vinelandii
2.3.1.12
-
 Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.2.1.104 reconstitution of pyruvate dehydrogenase complex Escherichia coli
1.2.1.104 reconstitution of pyruvate dehydrogenase complex Azotobacter vinelandii
2.3.1.12 reconstitution with components from Escherichia coli and Azotobacter vinelandii, optimum catalytic stoichiometry of E1:E2:E3 is 1:1:0.5 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Escherichia coli S-acetyldihydrolipoamide + CoA
-
 ?
2.3.1.12 dihydrolipoamide + acetyl-CoA
-
 Azotobacter vinelandii S-acetyldihydrolipoamide + CoA
-
 ?