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Literature summary extracted from
- Purification and characterization of thermostable maltooligosyl trehalose synthase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius (1996), Biosci. Biotechnol. Biochem., 60, 263-266.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.15 | Cu2+ | slight | Sulfolobus acidocaldarius |  |
| 5.4.99.15 | Hg2+ | strong | Sulfolobus acidocaldarius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 0.6 | - |
short chain amylose | - |
Sulfolobus acidocaldarius | |
| 5.4.99.15 | 1.4 | - |
maltohexaose | maltoheptaose | Sulfolobus acidocaldarius | |
| 5.4.99.15 | 5.7 | - |
maltohexaose | - |
Sulfolobus acidocaldarius | |
| 5.4.99.15 | 7.1 | - |
maltopentaose | - |
Sulfolobus acidocaldarius | |
| 5.4.99.15 | 41.5 | - |
maltotetraose | - |
Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 74000 | - |
x * 74000, SDS-PAGE | Sulfolobus acidocaldarius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.15 | Sulfolobus acidocaldarius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | - |
Sulfolobus acidocaldarius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 80.8 | - |
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.15 | Maltoheptaose | 92% of the activity with maltopentaose | Sulfolobus acidocaldarius | ? | - |
? | |
| 5.4.99.15 | Maltohexaose | 145% of the activity with maltopentaose | Sulfolobus acidocaldarius | ? | - |
? | |
| 5.4.99.15 | Maltopentaose | - |
Sulfolobus acidocaldarius | alpha-Maltotriosyl trehalose | - |
? | |
| 5.4.99.15 | Maltotetraose | 23% of the activity with maltopentaose | Sulfolobus acidocaldarius | ? | - |
? | |
| 5.4.99.15 | Short chain amylose | 43% of the activity with maltopentaose | Sulfolobus acidocaldarius | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | ? | x * 74000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 85 | - |
60 min, stable up to | Sulfolobus acidocaldarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 5 | 5.5 | - |
Sulfolobus acidocaldarius |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 4.5 | 9.5 | 4°C, 24 h, stable | Sulfolobus acidocaldarius |
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