EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | Chloral hydrate | inhibition of isoenzyme I and II from mitochondria and isoenzyme II from microsomes, no inhibition of isoenzyme I from microsomes | Rattus norvegicus | |
1.2.1.3 | Disulfiram | inhibits activity of mitochondrial isoenzyme I with NAD+ and activity of isoenzyme I and II from microsomes and mitochondria with NADP+ | Rattus norvegicus | |
1.2.1.3 | NAD+ | 0.1 mM, significant inhibition of esterase activity | Rattus norvegicus | |
1.2.1.3 | p-hydroxymercuribenzoate | 0.05 mM, potent inhibition of isoenzyme I from microsomes and mitochondria, isoenzyme II from microsomes and mitochondria is less sensitive | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.3 | 0.022 | - |
NAD+ | reaction with propanal, enzyme II from mitochondria | Rattus norvegicus | |
1.2.1.3 | 0.036 | - |
NAD+ | reaction with propanal, enzyme I from mitochondria | Rattus norvegicus | |
1.2.1.3 | 0.095 | - |
NAD+ | reaction with propanal, enzyme II from microsomes | Rattus norvegicus | |
1.2.1.3 | 0.097 | - |
NAD+ | reaction with propanal, enzyme I from microsomes | Rattus norvegicus | |
1.2.1.3 | 0.15 | - |
propanal | enzyme I and II from mitochondria | Rattus norvegicus | |
1.2.1.3 | 0.27 | - |
NADP+ | reaction with benzaldehyde, enzyme II from microsomes | Rattus norvegicus | |
1.2.1.3 | 0.44 | - |
NADP+ | reaction with benzaldehyde, enzyme II from mitochondria | Rattus norvegicus | |
1.2.1.3 | 0.44 | - |
NADP+ | reaction with benzaldehyde, enzyme I from microsomes | Rattus norvegicus | |
1.2.1.3 | 0.91 | - |
NADP+ | reaction with benzaldehyde, enzyme I from mitochondria | Rattus norvegicus | |
1.2.1.3 | 1.6 | - |
benzaldehyde | enzyme I from mitochondria | Rattus norvegicus | |
1.2.1.3 | 1.7 | - |
propanal | enzyme II from microsome | Rattus norvegicus | |
1.2.1.3 | 2.4 | - |
acetaldehyde | enzyme I from microsome | Rattus norvegicus | |
1.2.1.3 | 3 | - |
benzaldehyde | enzyme II from microsomes | Rattus norvegicus | |
1.2.1.3 | 5.9 | - |
benzaldehyde | enzyme I from microsomes | Rattus norvegicus | |
1.2.1.3 | 50 | - |
benzaldehyde | enzyme II from mitochondria | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.3 | microsome | isoenzyme I and II | Rattus norvegicus | - |
- |
1.2.1.3 | mitochondrion | isoenzyme I and II | Rattus norvegicus | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | Mg2+ | 0.5 mM, slight activation | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 54000 | - |
2 * 54000, SDS-PAGE | Rattus norvegicus |
1.2.1.3 | 170000 | - |
mitochondrial isoenzyme I, gel filtration | Rattus norvegicus |
1.2.1.3 | 250000 | - |
mitochondrial isoenzyme II, microsomal isoenzyme I and II, gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.3 | Rattus norvegicus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.3 | liver | - |
Rattus norvegicus | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.3 | -20°C, all isoenzymes are stable for up to 1 month | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.3 | acetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | acetate + NADH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NAD+ + H2O | - |
Rattus norvegicus | benzoate + NADH + H+ | - |
? | |
1.2.1.3 | benzaldehyde + NADP+ | - |
Rattus norvegicus | benzoate + NADPH | - |
? | |
1.2.1.3 | p-nitrophenyl acetate + H2O | esterase activity of the various isoenzymes is less than 7% of the activity with propanal and NAD+ | Rattus norvegicus | p-nitrophenol + acetate | - |
? | |
1.2.1.3 | phenylacetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | phenylacetate + NADH + H+ | - |
? | |
1.2.1.3 | propanal + NAD+ + H2O | - |
Rattus norvegicus | propionate + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.3 | dimer | 2 * 54000, SDS-PAGE | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 55 | - |
5 min, microsomal isoenzymes and mitochondrial isoenzyme I, more than 90% loss of activity. Mitochondrial isoenzyme II retains 20% of its original activity | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.3 | 8.8 | - |
microsomal isoenzyme I and II and mitochondrial isoenzymes with NADP+ | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.3 | NAD+ | all isoenzymes prefer NAD+ over NADP+ | Rattus norvegicus | |
1.2.1.3 | NADP+ | all isoenzymes prefer NAD+ over NADP+ | Rattus norvegicus |