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Literature summary extracted from

  • Guerrillot, L.; Vandecasteele, J.P.
    Purification and characterization of two aldehyde dehydrogenases from Pseudomonas aeruginosa (1977), Eur. J. Biochem., 81, 185-192.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 NH4+ slight activation Pseudomonas aeruginosa
1.2.1.3 NH4+ activation seems to be due to increase of ionic strength Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Iodosobenzoate 0.1 mM, 3 min, complete inhibition Pseudomonas aeruginosa
1.2.1.3 p-hydroxymercuribenzoate 0.1 mM, 3 min, complete inhibition Pseudomonas aeruginosa
1.2.1.4 o-Iodosobenzoate 1 mM, 3 min, complete inhibition Pseudomonas aeruginosa
1.2.1.4 p-hydroxymercuribenzoate 0.1 mM, 3 min, complete inhibition Pseudomonas aeruginosa
1.2.1.4 Sodium acetate 1 mM, 92% inhibition Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 additional information
-
 additional information influence of pH-value and buffer on Km-value for hexanal Pseudomonas aeruginosa
1.2.1.3 0.0095
-
 hexanal
-
 Pseudomonas aeruginosa
1.2.1.3 0.045
-
 Butanal
-
 Pseudomonas aeruginosa
1.2.1.3 0.064
-
 propanal
-
 Pseudomonas aeruginosa
1.2.1.3 0.165
-
 acetaldehyde
-
 Pseudomonas aeruginosa
1.2.1.4 additional information
-
 additional information influence of pH-value and buffer of the Km-value for hexanal Pseudomonas aeruginosa
1.2.1.4 0.01
-
 dodecanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.015
-
 Tetradecanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.016
-
 Undecanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.023
-
 decanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.023
-
 tridecanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.025
-
 Nonanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.062
-
 octanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.065
-
 NADP+ reaction with pentanal Pseudomonas aeruginosa
1.2.1.4 0.108
-
 Heptanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.25
-
 hexanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.435
-
 pentanal
-
 Pseudomonas aeruginosa
1.2.1.4 2.2
-
 Butanal
-
 Pseudomonas aeruginosa
1.2.1.4 11
-
 propanal
-
 Pseudomonas aeruginosa
1.2.1.4 35
-
 acetaldehyde
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 K+ slight activation Pseudomonas aeruginosa
1.2.1.3 K+ activation seems to be due to increase of ionic strength Pseudomonas aeruginosa
1.2.1.4 K+ activates Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 225000
-
 gel filtration Pseudomonas aeruginosa
1.2.1.4 215000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.3 additional information Pseudomonas aeruginosa constitutive enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Pseudomonas aeruginosa
-
-
-
1.2.1.4 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Pseudomonas aeruginosa
1.2.1.4
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 additional information
-
-
 Pseudomonas aeruginosa
1.2.1.4 additional information
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.2.1.3 -8°C, 16% glycerol, 1 mM dithiothreitol, 25% loss of activity after 6 months Pseudomonas aeruginosa
1.2.1.3 4°C, 60% loss of activity after 44 h in absence of glycerol, 10% loss of activity in presence of 16% glycerol Pseudomonas aeruginosa
1.2.1.4 -8°C, 16% glycerol, 1 mM dithiothreitol, 12% loss of activity after 6 months Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa acetate + NADH + H+
-
 ?
1.2.1.3 butanal + NAD+ + H2O
-
 Pseudomonas aeruginosa butyrate + NADH + H+
-
 ?
1.2.1.3 decanal + NAD+ + H2O
-
 Pseudomonas aeruginosa decanoate + NADH
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Pseudomonas aeruginosa hexanoate + NADH + H+
-
 ?
1.2.1.3 additional information constitutive enzyme Pseudomonas aeruginosa ?
-
 ?
1.2.1.3 octanal + NAD+ + H2O
-
 Pseudomonas aeruginosa octanoate + NADH
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Pseudomonas aeruginosa propionate + NADH
-
 ?
1.2.1.4 acetaldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa acetate + NADPH + H+
-
 ?
1.2.1.4 butanal + NADP+ + H2O
-
 Pseudomonas aeruginosa butyrate + NADPH + H+
-
 ?
1.2.1.4 decanal + NADP+
-
 Pseudomonas aeruginosa decanoate + NADPH
-
 ?
1.2.1.4 dodecanal + NADP+ + H2O
-
 Pseudomonas aeruginosa dodecanoate + NADPH + H+
-
 ?
1.2.1.4 formaldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa formate + NADPH + H+
-
 ?
1.2.1.4 heptanal + NADP+
-
 Pseudomonas aeruginosa heptanoate + NADPH
-
 ?
1.2.1.4 hexanal + NADP+
-
 Pseudomonas aeruginosa hexanoate + NADPH
-
 ?
1.2.1.4 nonanal + NADP+ + H2O
-
 Pseudomonas aeruginosa nonanoate + NADPH + H+
-
 r
1.2.1.4 octanal + NADP+ + H2O
-
 Pseudomonas aeruginosa octanoate + NADPH + H+
-
 ?
1.2.1.4 pentanal + NADP+
-
 Pseudomonas aeruginosa pentanoate + NADPH
-
 ?
1.2.1.4 propanal + NADP+ + H2O
-
 Pseudomonas aeruginosa propionate + NADPH + H+
-
 ?
1.2.1.4 tetradecanal + NADP+
-
 Pseudomonas aeruginosa tetradecanoate + NADPH
-
 ?
1.2.1.4 tridecanal + NADP+
-
 Pseudomonas aeruginosa tridecanoate + NADPH
-
 ?
1.2.1.4 undecanal + NADP+
-
 Pseudomonas aeruginosa undecanoate + NADPH
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.3 7.4
-
 30°C, stable Pseudomonas aeruginosa
1.2.1.3 8.6
-
 30°C, rapid inactivation, NAD+ protects Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Pseudomonas aeruginosa
1.2.1.4 NADP+
-
 Pseudomonas aeruginosa