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Literature summary extracted from

  • Greenfield, N.J.; Pietruszko, R.
    Two aldehyde dehydrogenases from human liver. Isolation via affinity chromatography and characterization of the isozymes (1977), Biochim. Biophys. Acta, 483, 35-45.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.3 freezing and thawing causes loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.3 Disulfiram
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0001
-
 acetaldehyde isoenzyme 1 Homo sapiens
1.2.1.3 0.0007
-
 propanal isoenzyme 2, pH 7.0 Homo sapiens
1.2.1.3 0.0012
-
 propanal isoenzyme 2, pH 9.5 Homo sapiens
1.2.1.3 0.0024
-
 acetaldehyde isoenzyme 2, pH 9.5 Homo sapiens
1.2.1.3 0.003
-
 acetaldehyde isoenzyme 2, pH 7.0 Homo sapiens
1.2.1.3 0.008
-
 NAD+ isoenzyme 1, pH 9.5 Homo sapiens
1.2.1.3 0.011
-
 propanal isoenzyme 2, pH 9.5 Homo sapiens
1.2.1.3 0.04
-
 NAD+ isoenzyme 1, pH 7.0 Homo sapiens
1.2.1.3 0.07
-
 NAD+ isoenzyme 2, pH 7.0 and pH 9.5 Homo sapiens
1.2.1.3 0.12
-
 acetaldehyde isoenzyme 1, pH 9.5 Homo sapiens
1.2.1.3 1
-
 propanal isoenzyme 1, pH 7.0 Homo sapiens
1.2.1.3 2.5
-
 acetaldehyde isoenzyme 1, pH 7.0 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 54200
-
 4 * 54200, isoenzyme 2, SDS-PAGE Homo sapiens
1.2.1.3 54800
-
 4 * 54800, isoenzyme 1, SDS-PAGE Homo sapiens
1.2.1.3 225000
-
 enzyme 2, non-denaturing PAGE Homo sapiens
1.2.1.3 245000
-
 enzyme 1, non-denaturing PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.3 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 0.58
-
 isoenzyme 1 Homo sapiens
1.2.1.3 1.01
-
 isoenzyme E2 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
1.2.1.3 propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 tetramer 4 * 54800, isoenzyme 1, SDS-PAGE Homo sapiens
1.2.1.3 tetramer 4 * 54200, isoenzyme 2, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.3 8.6
-
 isoenzyme 1 Homo sapiens
1.2.1.3 9
-
 above, isoenzyme 2 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.3 7 9 pH 7.0: about 66% of maximal activity, pH 9.0: about 85% of maximal activity, isoenzyme 1 Homo sapiens
1.2.1.3 8 9.5 pH 8.0: about 35% of maximal activity, above pH 9.0: optimum, isoenzyme 2 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.3 0.0002
-
 Disulfiram isoenzyme 1 Homo sapiens