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Literature summary extracted from

  • Farres, J.; Wang, X.; Takahashi, K.; Cunningham, S.J.; Wang, T.T.; Weiner, H.
    Effects of changing glutamate 487 to lysine in rat and human liver mitochondrial aldehyde dehydrogenase. A model to study human (Oriental type) class 2 aldehyde dehydrogenase (1994), J. Biol. Chem., 269, 13854-13860.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 mutant enzymes expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.3 E487K mutant enzyme shows altered kinetic properties when compared to wild-type enzyme, The Km-value for NAD+ at pH 7.4 increases more than 150fold, the turnover-number decreases 2-10fold, many oriental people posses this variant of liver mitochondrial aldehyde dehydrogenase and have very low levels of enzyme activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.00008
-
propanal native enzyme, pH 7.4 Rattus norvegicus
1.2.1.3 0.0001
-
propanal native enzyme, pH 9.0 Rattus norvegicus
1.2.1.3 0.0002
-
propanal mutant enzyme E487Q, pH 7.4 Homo sapiens
1.2.1.3 0.0006
-
propanal mutant enzyme E487K, pH 7.4 Rattus norvegicus
1.2.1.3 0.0007
-
propanal
-
Rattus norvegicus
1.2.1.3 0.0007
-
propanal native enzyme, pH 7.4 Homo sapiens
1.2.1.3 0.0014
-
propanal mutant enzyme E487K, pH 7.4 Homo sapiens
1.2.1.3 0.0017
-
propanal mutant enzyme E487K, pH 9.0 Rattus norvegicus
1.2.1.3 0.004
-
NAD+
-
Homo sapiens
1.2.1.3 0.004
-
NAD+ native enzyme, pH 7.4 Rattus norvegicus
1.2.1.3 0.07
-
NAD+ native enzyme, pH 7.4 Homo sapiens
1.2.1.3 0.074
-
NAD+ native enzyme, pH 9.0 Rattus norvegicus
1.2.1.3 0.09
-
NAD+ mutant enzyme E487Q Homo sapiens
1.2.1.3 0.7
-
NAD+ mutant enzyme E487K, pH 7.4 Rattus norvegicus
1.2.1.3 1.1
-
NAD+ mutant enzyme E487K, pH 9.0 Rattus norvegicus
1.2.1.3 7.4
-
NAD+ mutant enzyme E487K Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Homo sapiens
-
-
-
1.2.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
Homo sapiens
1.2.1.3
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
Homo sapiens acetate + NADH + H+
-
?
1.2.1.3 acetaldehyde + NAD+ + H2O
-
Rattus norvegicus acetate + NADH + H+
-
?
1.2.1.3 chloroacetaldehyde + NAD+ + H2O activity is twice as fast as with acetaldehyde, native enzyme and mutant enzyme E487K Homo sapiens chloroacetate + NADH + H+
-
?
1.2.1.3 chloroacetaldehyde + NAD+ + H2O activity is twice as fast as with acetaldehyde, native enzyme and mutant enzyme E487K Rattus norvegicus chloroacetate + NADH + H+
-
?
1.2.1.3 propanal + NAD+ + H2O
-
Homo sapiens propionate + NADH
-
?
1.2.1.3 propanal + NAD+ + H2O
-
Rattus norvegicus propionate + NADH
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.3 additional information
-
additional information
-
Homo sapiens
1.2.1.3 additional information
-
additional information
-
Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
Homo sapiens
1.2.1.3 NAD+
-
Rattus norvegicus