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Literature summary extracted from

  • Vandecasteele, J.P.; Guerrillot, L.
    Aldehyde dehydrogenases from Pseudomonas aeruginosa (1982), Methods Enzymol., 89, 484-490.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 NH4+ slight activation Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.0013
-
 decanal
-
 Pseudomonas aeruginosa
1.2.1.3 0.0095
-
 hexanal
-
 Pseudomonas aeruginosa
1.2.1.3 0.044
-
 NAD+ reaction with butanal Pseudomonas aeruginosa
1.2.1.3 0.045
-
 Butanal
-
 Pseudomonas aeruginosa
1.2.1.3 0.165
-
 acetaldehyde
-
 Pseudomonas aeruginosa
1.2.1.4 0.015
-
 Tetradecanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.065
-
 NADP+ reaction with pentanal Pseudomonas aeruginosa
1.2.1.4 0.25
-
 hexanal
-
 Pseudomonas aeruginosa
1.2.1.4 0.435
-
 pentanal
-
 Pseudomonas aeruginosa
1.2.1.4 35
-
 acetaldehyde
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 K+ slight activation Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 225000
-
 gel filtration Pseudomonas aeruginosa
1.2.1.4 215000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Pseudomonas aeruginosa
-
-
-
1.2.1.4 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3
-
 Pseudomonas aeruginosa
1.2.1.4
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.3 20
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.2.1.4 -8°C, 12% loss of activity after 6 months Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 acetaldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa acetate + NADH + H+
-
 ?
1.2.1.3 butanal + NAD+ + H2O
-
 Pseudomonas aeruginosa butyrate + NADH + H+
-
 ?
1.2.1.3 decanal + NAD+ + H2O
-
 Pseudomonas aeruginosa decanoate + NADH
-
 ?
1.2.1.3 hexanal + NAD+ + H2O
-
 Pseudomonas aeruginosa hexanoate + NADH + H+
-
 ?
1.2.1.4 acetaldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa acetate + NADPH + H+
-
 ?
1.2.1.4 heptanal + NADP+
-
 Pseudomonas aeruginosa heptanoate + NADPH
-
 ?
1.2.1.4 hexanal + NADP+
-
 Pseudomonas aeruginosa hexanoate + NADPH
-
 ?
1.2.1.4 pentanal + NADP+
-
 Pseudomonas aeruginosa pentanoate + NADPH
-
 ?
1.2.1.4 tetradecanal + NADP+
-
 Pseudomonas aeruginosa tetradecanoate + NADPH
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Pseudomonas aeruginosa
1.2.1.4 NADP+
-
 Pseudomonas aeruginosa