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Literature summary extracted from

  • Nakamura, K.; Bernheim, F.
    Studies on malonic semialdehyde dehydrogenase from Pseudomonas aeruginosa (1961), Biochim. Biophys. Acta, 50, 147-152.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.15 1 mM mercaptoethanol is essential for stability Pseudomonas aeruginosa
1.2.1.15 unstable in dilute solution Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.15 Cd2+
-
 Pseudomonas aeruginosa
1.2.1.15 Cu2+
-
 Pseudomonas aeruginosa
1.2.1.15 glyoxylate competitive inhibitor Pseudomonas aeruginosa
1.2.1.15 HgCl2
-
 Pseudomonas aeruginosa
1.2.1.15 malonate competitive inhibitor Pseudomonas aeruginosa
1.2.1.15 Ni2+
-
 Pseudomonas aeruginosa
1.2.1.15 p-chloromercuribenzoate
-
 Pseudomonas aeruginosa
1.2.1.15 phosphate
-
 Pseudomonas aeruginosa
1.2.1.15 succinate semialdehyde non-competitive inhibitor Pseudomonas aeruginosa
1.2.1.15 Zn2+
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.15 0.043
-
 malonate semialdehyde
-
 Pseudomonas aeruginosa
1.2.1.15 0.11
-
 NAD+
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.15 Ca2+ increases activity 42% at 1 mM Pseudomonas aeruginosa
1.2.1.15 Mg2+ increases activity 64% at 1 mM Pseudomonas aeruginosa
1.2.1.15 Mn2+ increases activity 27% at 1 mM Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.15 3-oxopropanoate + NAD+ + H2O Pseudomonas aeruginosa
-
 malonate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.15 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.15
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.15 0.203
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.2.1.15 -20°C, purified enzyme, 1 month Pseudomonas aeruginosa
1.2.1.15 0°C, bovine serum albumin, NAD+, 1 week Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.15 3-oxopropanoate + NAD+ + H2O
-
 Pseudomonas aeruginosa malonate + NADH
-
 ?
1.2.1.15 additional information no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde Pseudomonas aeruginosa ?
-
 ?
1.2.1.15 NAD(P)+ + 3-oxopropanoate + H2O
-
 Pseudomonas aeruginosa malonate + NAD(P)H
-
 ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.15 55
-
 with NAD+ and bovine serum albumin at pH 8.7 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.15 8.7
-
 malonate semialdehyde + NAD+ Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.15 NAD+
-
 Pseudomonas aeruginosa
1.2.1.15 NADP+ less than 10% activity compared with NAD+ Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.15 0.56
-
 succinate semialdehyde
-
 Pseudomonas aeruginosa
1.2.1.15 0.57
-
 malonate
-
 Pseudomonas aeruginosa
1.2.1.15 1.8
-
 glyoxylate
-
 Pseudomonas aeruginosa