BRENDA - Enzyme Database

Studies on malonic semialdehyde dehydrogenase from Pseudomonas aeruginosa

Nakamura, K.; Bernheim, F.; Biochim. Biophys. Acta 50, 147-152 (1961)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.2.1.15
1 mM mercaptoethanol is essential for stability
Pseudomonas aeruginosa
1.2.1.15
unstable in dilute solution
Pseudomonas aeruginosa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.15
Cd2+
-
Pseudomonas aeruginosa
1.2.1.15
Cu2+
-
Pseudomonas aeruginosa
1.2.1.15
glyoxylate
competitive inhibitor
Pseudomonas aeruginosa
1.2.1.15
HgCl2
-
Pseudomonas aeruginosa
1.2.1.15
malonate
competitive inhibitor
Pseudomonas aeruginosa
1.2.1.15
Ni2+
-
Pseudomonas aeruginosa
1.2.1.15
p-chloromercuribenzoate
-
Pseudomonas aeruginosa
1.2.1.15
phosphate
-
Pseudomonas aeruginosa
1.2.1.15
succinate semialdehyde
non-competitive inhibitor
Pseudomonas aeruginosa
1.2.1.15
Zn2+
-
Pseudomonas aeruginosa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.15
0.043
-
Malonate semialdehyde
-
Pseudomonas aeruginosa
1.2.1.15
0.11
-
NAD+
-
Pseudomonas aeruginosa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.1.15
Ca2+
increases activity 42% at 1 mM
Pseudomonas aeruginosa
1.2.1.15
Mg2+
increases activity 64% at 1 mM
Pseudomonas aeruginosa
1.2.1.15
Mn2+
increases activity 27% at 1 mM
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.15
3-oxopropanoate + NAD+ + H2O
Pseudomonas aeruginosa
-
malonate + NADH
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.15
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.15
-
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.15
0.203
-
-
Pseudomonas aeruginosa
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.15
-20°C, purified enzyme, 1 month
Pseudomonas aeruginosa
1.2.1.15
0°C, bovine serum albumin, NAD+, 1 week
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.15
3-oxopropanoate + NAD+ + H2O
-
348638
Pseudomonas aeruginosa
malonate + NADH
-
-
-
-
1.2.1.15
additional information
no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde
348638
Pseudomonas aeruginosa
?
-
-
-
-
1.2.1.15
NAD(P)+ + 3-oxopropanoate + H2O
-
348638
Pseudomonas aeruginosa
malonate + NAD(P)H
-
348638
Pseudomonas aeruginosa
ir
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.15
55
-
with NAD+ and bovine serum albumin at pH 8.7
Pseudomonas aeruginosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.15
8.7
-
malonate semialdehyde + NAD+
Pseudomonas aeruginosa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.15
NAD+
-
Pseudomonas aeruginosa
1.2.1.15
NADP+
less than 10% activity compared with NAD+
Pseudomonas aeruginosa
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.15
0.56
-
succinate semialdehyde
-
Pseudomonas aeruginosa
1.2.1.15
0.57
-
malonate
-
Pseudomonas aeruginosa
1.2.1.15
1.8
-
glyoxylate
-
Pseudomonas aeruginosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.15
NAD+
-
Pseudomonas aeruginosa
1.2.1.15
NADP+
less than 10% activity compared with NAD+
Pseudomonas aeruginosa
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.15
1 mM mercaptoethanol is essential for stability
Pseudomonas aeruginosa
1.2.1.15
unstable in dilute solution
Pseudomonas aeruginosa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.15
Cd2+
-
Pseudomonas aeruginosa
1.2.1.15
Cu2+
-
Pseudomonas aeruginosa
1.2.1.15
glyoxylate
competitive inhibitor
Pseudomonas aeruginosa
1.2.1.15
HgCl2
-
Pseudomonas aeruginosa
1.2.1.15
malonate
competitive inhibitor
Pseudomonas aeruginosa
1.2.1.15
Ni2+
-
Pseudomonas aeruginosa
1.2.1.15
p-chloromercuribenzoate
-
Pseudomonas aeruginosa
1.2.1.15
phosphate
-
Pseudomonas aeruginosa
1.2.1.15
succinate semialdehyde
non-competitive inhibitor
Pseudomonas aeruginosa
1.2.1.15
Zn2+
-
Pseudomonas aeruginosa
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.15
0.56
-
succinate semialdehyde
-
Pseudomonas aeruginosa
1.2.1.15
0.57
-
malonate
-
Pseudomonas aeruginosa
1.2.1.15
1.8
-
glyoxylate
-
Pseudomonas aeruginosa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.15
0.043
-
Malonate semialdehyde
-
Pseudomonas aeruginosa
1.2.1.15
0.11
-
NAD+
-
Pseudomonas aeruginosa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.1.15
Ca2+
increases activity 42% at 1 mM
Pseudomonas aeruginosa
1.2.1.15
Mg2+
increases activity 64% at 1 mM
Pseudomonas aeruginosa
1.2.1.15
Mn2+
increases activity 27% at 1 mM
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.15
3-oxopropanoate + NAD+ + H2O
Pseudomonas aeruginosa
-
malonate + NADH
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.15
-
Pseudomonas aeruginosa
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.15
0.203
-
-
Pseudomonas aeruginosa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.15
-20°C, purified enzyme, 1 month
Pseudomonas aeruginosa
1.2.1.15
0°C, bovine serum albumin, NAD+, 1 week
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.15
3-oxopropanoate + NAD+ + H2O
-
348638
Pseudomonas aeruginosa
malonate + NADH
-
-
-
-
1.2.1.15
additional information
no activity with formaldehyde, acetaldehyde, propionaldehyde, isovaleraldehyde, benzaldehyde, anisaldehyde, glyceraldehyde, glyceraldehyde-3-phosphate, glyoxylate, succinic semialdehyde
348638
Pseudomonas aeruginosa
?
-
-
-
-
1.2.1.15
NAD(P)+ + 3-oxopropanoate + H2O
-
348638
Pseudomonas aeruginosa
malonate + NAD(P)H
-
348638
Pseudomonas aeruginosa
ir
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.15
55
-
with NAD+ and bovine serum albumin at pH 8.7
Pseudomonas aeruginosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.15
8.7
-
malonate semialdehyde + NAD+
Pseudomonas aeruginosa