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Literature summary extracted from

  • Nakada, T.; Maruta, K.; Tsusaki, K.; Kubota, M.; Chaen, H.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.
    Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36 (1995), Biosci. Biotechnol. Biochem., 59, 2210-2214.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.15 Cu2+ CuSO4 Arthrobacter sp.
5.4.99.15 FeSO4
-
 Arthrobacter sp.
5.4.99.15 Hg2+ HgCl2 Arthrobacter sp.
5.4.99.15 MgCl2 weak Arthrobacter sp.
5.4.99.15 ZnSO4 weak Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.15 0.9
-
 maltoheptaose
-
 Arthrobacter sp.
5.4.99.15 1.4
-
 maltohexaose
-
 Arthrobacter sp.
5.4.99.15 8.7
-
 maltopentaose
-
 Arthrobacter sp.
5.4.99.15 22.9
-
 maltotetraose
-
 Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.15 81000
-
 x * 81000, SDS-PAGE Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.15 Arthrobacter sp.
-
 Q36
-
5.4.99.15 Arthrobacter sp. Q36
-
 Q36
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.15
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.15 203
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 Maltoheptaose 58% of the activity with maltopentaose Arthrobacter sp. ?
-
 ?
5.4.99.15 Maltoheptaose 58% of the activity with maltopentaose Arthrobacter sp. Q36 ?
-
 ?
5.4.99.15 Maltohexaose 45% of the activity with maltopentaose Arthrobacter sp. ?
-
 ?
5.4.99.15 Maltohexaose 45% of the activity with maltopentaose Arthrobacter sp. Q36 ?
-
 ?
5.4.99.15 Maltopentaose
-
 Arthrobacter sp. alpha-Maltotriosyl trehalose
-
 ?
5.4.99.15 Maltopentaose
-
 Arthrobacter sp. Q36 alpha-Maltotriosyl trehalose
-
 ?
5.4.99.15 Maltotetraose 25% of the activity with maltopentaose Arthrobacter sp. ?
-
 ?
5.4.99.15 Maltotetraose 25% of the activity with maltopentaose Arthrobacter sp. Q36 ?
-
 ?
5.4.99.15 additional information no reaction with maltose Arthrobacter sp. ?
-
 ?
5.4.99.15 additional information no reaction with maltose Arthrobacter sp. Q36 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.15 ? x * 81000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.15 40
-
-
 Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.99.15 30 45 30°C: about 80% of maximal activity, 45°C: about 85% of maximal activity Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.15 40
-
 60 min, stable up to Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.15 7
-
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.15 5.5 8 pH 5.5: about 80% of maximal activity, pH 8.0: about 60% of maximal activity Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.15 6 9.5 4°C, 24 h, stable Arthrobacter sp.