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Literature summary extracted from

  • Hastings, J.W.; Baldwin, T.O.; Nicoli, M.Z.
    Bacterial luciferase: Assay, purification, and properties (1978), Methods Enzymol., 57, 135-152.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.14.3 citrate stabilizes against inactivation by proteases, heat, urea Aliivibrio fischeri
1.14.14.3 citrate stabilizes against inactivation by proteases, heat, urea Photobacterium phosphoreum
1.14.14.3 citrate stabilizes against inactivation by proteases, heat, urea Vibrio harveyi
1.14.14.3 diphosphate stabilizes against inactivation by proteases, heat, urea Aliivibrio fischeri
1.14.14.3 diphosphate stabilizes against inactivation by proteases, heat, urea Photobacterium phosphoreum
1.14.14.3 diphosphate stabilizes against inactivation by proteases, heat, urea Vibrio harveyi
1.14.14.3 inactivation by lyophilization Aliivibrio fischeri
1.14.14.3 inactivation by lyophilization Photobacterium phosphoreum
1.14.14.3 inactivation by lyophilization Vibrio harveyi
1.14.14.3 labile to proteases Aliivibrio fischeri
1.14.14.3 labile to proteases Photobacterium phosphoreum
1.14.14.3 labile to proteases Vibrio harveyi
1.14.14.3 no inactivation by repeated freezing/thawing Aliivibrio fischeri
1.14.14.3 no inactivation by repeated freezing/thawing Photobacterium phosphoreum
1.14.14.3 no inactivation by repeated freezing/thawing Vibrio harveyi
1.14.14.3 phosphate stabilizes against inactivation by proteases, heat, urea Aliivibrio fischeri
1.14.14.3 phosphate stabilizes against inactivation by proteases, heat, urea Photobacterium phosphoreum
1.14.14.3 phosphate stabilizes against inactivation by proteases, heat, urea Vibrio harveyi
1.14.14.3 sulfate stabilizes against inactivation by proteases, heat, urea Aliivibrio fischeri
1.14.14.3 sulfate stabilizes against inactivation by proteases, heat, urea Photobacterium phosphoreum
1.14.14.3 sulfate stabilizes against inactivation by proteases, heat, urea Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.3 2,3-Dichloro-(6-phenylphenoxy)ethylamine
-
Aliivibrio fischeri
1.14.14.3 2,3-Dichloro-(6-phenylphenoxy)ethylamine
-
Vibrio harveyi
1.14.14.3 2-diethylaminoethyl-2,2-diphenylvalerate
-
Aliivibrio fischeri
1.14.14.3 2-diethylaminoethyl-2,2-diphenylvalerate
-
Vibrio harveyi
1.14.14.3 8-Anilino-1-naphthalenesulfonate
-
Aliivibrio fischeri
1.14.14.3 8-Anilino-1-naphthalenesulfonate
-
Vibrio harveyi
1.14.14.3 amino group reagents
-
Aliivibrio fischeri
1.14.14.3 amino group reagents
-
Photobacterium phosphoreum
1.14.14.3 amino group reagents
-
Vibrio harveyi
1.14.14.3 imidazole reagents
-
Aliivibrio fischeri
1.14.14.3 imidazole reagents
-
Photobacterium phosphoreum
1.14.14.3 imidazole reagents
-
Vibrio harveyi
1.14.14.3 sulfhydryl reagents
-
Aliivibrio fischeri
1.14.14.3 sulfhydryl reagents
-
Photobacterium phosphoreum
1.14.14.3 sulfhydryl reagents
-
Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 decanal + FMNH + O2 Aliivibrio fischeri
-
decanoic acid + FMN + H2O + light
-
ir
1.14.14.3 decanal + FMNH + O2 Photobacterium phosphoreum
-
decanoic acid + FMN + H2O + light
-
ir
1.14.14.3 decanal + FMNH + O2 Vibrio harveyi
-
decanoic acid + FMN + H2O + light
-
ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Aliivibrio fischeri
-
formerly Photobacterium fischeri
-
1.14.14.3 Photobacterium phosphoreum
-
-
-
1.14.14.3 Vibrio harveyi
-
formerly Beneckea harveyi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.3
-
Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 decanal + FMNH + O2
-
Aliivibrio fischeri decanoic acid + FMN + H2O + light
-
ir
1.14.14.3 decanal + FMNH + O2
-
Photobacterium phosphoreum decanoic acid + FMN + H2O + light
-
ir
1.14.14.3 decanal + FMNH + O2
-
Vibrio harveyi decanoic acid + FMN + H2O + light
-
ir
1.14.14.3 dodecanal + FMNH + O2
-
Aliivibrio fischeri dodecanoic acid + FMN + H2O + light
-
ir
1.14.14.3 dodecanal + FMNH + O2
-
Photobacterium phosphoreum dodecanoic acid + FMN + H2O + light
-
ir
1.14.14.3 dodecanal + FMNH + O2
-
Vibrio harveyi dodecanoic acid + FMN + H2O + light
-
ir
1.14.14.3 additional information aldehydes of chain-length 8 or more required Aliivibrio fischeri ?
-
?
1.14.14.3 additional information aldehydes of chain-length 8 or more required Photobacterium phosphoreum ?
-
?
1.14.14.3 additional information aldehydes of chain-length 8 or more required Vibrio harveyi ?
-
?
1.14.14.3 octanal + FMNH + O2
-
Aliivibrio fischeri octanoic acid + FMN + H2O + light
-
ir
1.14.14.3 octanal + FMNH + O2
-
Photobacterium phosphoreum octanoic acid + FMN + H2O + light
-
ir
1.14.14.3 octanal + FMNH + O2
-
Vibrio harveyi octanoic acid + FMN + H2O + light
-
ir

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.3 35
-
stable below Aliivibrio fischeri
1.14.14.3 35
-
stable below Photobacterium phosphoreum
1.14.14.3 35
-
stable below Vibrio harveyi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.14.3 6 8.5
-
Aliivibrio fischeri
1.14.14.3 6 9.5
-
Vibrio harveyi