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Literature summary extracted from

  • Escher, A.; O'Kane, D.J.; Lee, J.; Szalay, A.A.
    Bacterial luciferase alpha beta fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature (1989), Proc. Natl. Acad. Sci. USA, 86, 6528-6532.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.3 78000
-
 1 * 78000, produced by gene fusion of luxA and luxB genes Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.3 decanal + FMNH + O2 Vibrio harveyi
-
 decanoic acid + FMN + H2O + light
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio harveyi
-
 expressed in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 decanal + FMNH + O2
-
 Vibrio harveyi decanoic acid + FMN + H2O + light
-
 ir
1.14.14.3 dodecanal + FMNH + O2
-
 Vibrio harveyi dodecanoic acid + FMN + H2O + light
-
 ir
1.14.14.3 octanal + FMNH + O2
-
 Vibrio harveyi octanoic acid + FMN + H2O + light
-
 ir

Subunits

EC Number Subunits Comment Organism
1.14.14.3 monomer 1 * 78000, produced by gene fusion of luxA and luxB genes Vibrio harveyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.3 37
-
 comparison of stability of wild-type enzyme and gene-fusion monomeric enzyme Vibrio harveyi