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Literature summary extracted from
- Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium (1990), J. Biol. Chem., 265, 16581-16587.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.3 | - |
Vibrio harveyi |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.3 | 77000 | 78000 | - |
Vibrio harveyi |
| 1.14.14.3 | 77000 | 78000 | gel filtration | Photorhabdus luminescens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.3 | decanal + FMNH + O2 | Vibrio harveyi | - |
decanoic acid + FMN + H2O + light | - |
ir |  |
| 1.14.14.3 | decanal + FMNH + O2 | Photorhabdus luminescens | - |
decanoic acid + FMN + H2O + light | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.3 | Photorhabdus luminescens | - |
- |
- |
| 1.14.14.3 | Vibrio harveyi | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.3 | decanal + FMNH + O2 | - |
Vibrio harveyi | decanoic acid + FMN + H2O + light | - |
ir | |
| 1.14.14.3 | decanal + FMNH + O2 | - |
Photorhabdus luminescens | decanoic acid + FMN + H2O + light | - |
ir | |
| 1.14.14.3 | dodecanal + FMNH + O2 | - |
Vibrio harveyi | dodecanoic acid + FMN + H2O + light | - |
ir | |
| 1.14.14.3 | dodecanal + FMNH + O2 | - |
Photorhabdus luminescens | dodecanoic acid + FMN + H2O + light | - |
ir | |
| 1.14.14.3 | octanal + FMNH + O2 | - |
Vibrio harveyi | octanoic acid + FMN + H2O + light | - |
ir | |
| 1.14.14.3 | octanal + FMNH + O2 | - |
Photorhabdus luminescens | octanoic acid + FMN + H2O + light | - |
ir | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.3 | 45 | - |
half-life over 3 h | Photorhabdus luminescens |
| 1.14.14.3 | 45 | - |
half-life 5 min | Vibrio harveyi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.3 | additional information | - |
additional information | - |
Vibrio harveyi | |
| 1.14.14.3 | additional information | - |
additional information | tetradecanal + FMNH + O2 (possibly) turnover rate determined by measuring the half-time for decay of luminescence between 70% and 35% of the maximum intensity, values depending on chain-length of aldehyde | Photorhabdus luminescens |
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Release 2023.1 (January 2023)
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