BRENDA - Enzyme Database

Kynurenine 3-hydroxylase in brain: species activity differences and effect of gerbil cerebral ischemia

Saito, K.; Quearry, B.J.; Saito, M.; Nowak, T.S.Jr.; Markey S.P.; Heyes, M.P.; Arch. Biochem. Biophys. 307, 104-109 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.9
anthranilic acid
22% inhibition at 2 mM and 11% inhibition at 0.2 mM
Meriones unguiculatus
1.14.13.9
EDTA
68% inhibition at 2 mM
Meriones unguiculatus
1.14.13.9
Kynurenic acid
13% inhibition at 2 mM and 5% inhibition at 0.2 mM
Meriones unguiculatus
1.14.13.9
L-tryptophan
35% inhibition at 2 mM and 22% inhibition at 0.2 mM
Meriones unguiculatus
1.14.13.9
xanthurenic acid
48% inhibition at 2 mM and 13% inhibition at 0.2 mM
Meriones unguiculatus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.9
0.0164
-
L-kynurenine
-
Meriones unguiculatus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.9
L-kynurenine + NADPH + O2
Rattus norvegicus
-
? + NADP+
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
Meriones unguiculatus
increased activity in injured brain regions following cerebral ischemia
? + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.9
Homo sapiens
-
-
-
1.14.13.9
Macaca mulatta
-
male rhesus macaques
-
1.14.13.9
Meriones unguiculatus
-
female mongolian gerbil
-
1.14.13.9
Mus musculus
-
mouse, strain D57Bl/6J
-
1.14.13.9
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.9
brain
-
Homo sapiens
-
1.14.13.9
brain
-
Macaca mulatta
-
1.14.13.9
brain
-
Meriones unguiculatus
-
1.14.13.9
brain
-
Rattus norvegicus
-
1.14.13.9
intestine
-
Meriones unguiculatus
-
1.14.13.9
kidney
-
Meriones unguiculatus
-
1.14.13.9
kidney
-
Rattus norvegicus
-
1.14.13.9
liver
-
Meriones unguiculatus
-
1.14.13.9
liver
-
Rattus norvegicus
-
1.14.13.9
lung
-
Meriones unguiculatus
-
1.14.13.9
spleen
-
Meriones unguiculatus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.9
additional information
-
-
Homo sapiens
1.14.13.9
additional information
-
-
Macaca mulatta
1.14.13.9
additional information
-
specific activity in various organs
Meriones unguiculatus
1.14.13.9
additional information
-
-
Mus musculus
1.14.13.9
additional information
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
1.14.13.9
-70°C no loss of enzymatic activity after 24 hours
Meriones unguiculatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.9
L-kynurenine + NADPH + O2
-
348521
Rattus norvegicus
3-hydroxy-L-kynurenine + NADP+ + H2O
-
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
-
348521
Meriones unguiculatus
3-hydroxy-L-kynurenine + NADP+ + H2O
-
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
-
348521
Rattus norvegicus
? + NADP+
-
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
increased activity in injured brain regions following cerebral ischemia
348521
Meriones unguiculatus
? + NADP+
-
-
-
?
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.13.9
7
10
50% activity at 7°C and 10°C
Meriones unguiculatus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.13.9
4
-
no loss of enzymatic activity after 24 hours
Meriones unguiculatus
1.14.13.9
25
-
22% loss of enzymatic activity after 24 hours
Meriones unguiculatus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.9
8
8.5
-
Meriones unguiculatus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.13.9
anthranilic acid
22% inhibition at 2 mM and 11% inhibition at 0.2 mM
Meriones unguiculatus
1.14.13.9
EDTA
68% inhibition at 2 mM
Meriones unguiculatus
1.14.13.9
Kynurenic acid
13% inhibition at 2 mM and 5% inhibition at 0.2 mM
Meriones unguiculatus
1.14.13.9
L-tryptophan
35% inhibition at 2 mM and 22% inhibition at 0.2 mM
Meriones unguiculatus
1.14.13.9
xanthurenic acid
48% inhibition at 2 mM and 13% inhibition at 0.2 mM
Meriones unguiculatus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.13.9
0.0164
-
L-kynurenine
-
Meriones unguiculatus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.9
L-kynurenine + NADPH + O2
Rattus norvegicus
-
? + NADP+
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
Meriones unguiculatus
increased activity in injured brain regions following cerebral ischemia
? + NADP+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.9
brain
-
Homo sapiens
-
1.14.13.9
brain
-
Macaca mulatta
-
1.14.13.9
brain
-
Meriones unguiculatus
-
1.14.13.9
brain
-
Rattus norvegicus
-
1.14.13.9
intestine
-
Meriones unguiculatus
-
1.14.13.9
kidney
-
Meriones unguiculatus
-
1.14.13.9
kidney
-
Rattus norvegicus
-
1.14.13.9
liver
-
Meriones unguiculatus
-
1.14.13.9
liver
-
Rattus norvegicus
-
1.14.13.9
lung
-
Meriones unguiculatus
-
1.14.13.9
spleen
-
Meriones unguiculatus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.13.9
additional information
-
-
Homo sapiens
1.14.13.9
additional information
-
-
Macaca mulatta
1.14.13.9
additional information
-
specific activity in various organs
Meriones unguiculatus
1.14.13.9
additional information
-
-
Mus musculus
1.14.13.9
additional information
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.13.9
-70°C no loss of enzymatic activity after 24 hours
Meriones unguiculatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.9
L-kynurenine + NADPH + O2
-
348521
Rattus norvegicus
3-hydroxy-L-kynurenine + NADP+ + H2O
-
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
-
348521
Meriones unguiculatus
3-hydroxy-L-kynurenine + NADP+ + H2O
-
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
-
348521
Rattus norvegicus
? + NADP+
-
-
-
?
1.14.13.9
L-kynurenine + NADPH + O2
increased activity in injured brain regions following cerebral ischemia
348521
Meriones unguiculatus
? + NADP+
-
-
-
?
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.13.9
7
10
50% activity at 7°C and 10°C
Meriones unguiculatus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.13.9
4
-
no loss of enzymatic activity after 24 hours
Meriones unguiculatus
1.14.13.9
25
-
22% loss of enzymatic activity after 24 hours
Meriones unguiculatus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.9
8
8.5
-
Meriones unguiculatus