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Literature summary extracted from

  • Wiesner, W.; van Pee, K.H.; Lingens, F.
    Purification and characterization of a novel bacterial non-heme chloroperoxidase from Pseudomonas pyrrocinia (1988), J. Biol. Chem., 263, 13725-13732.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.B2 azide no inhibition Burkholderia pyrrocinia
1.11.1.B2 F- 0.18 mM, 50% inhibition Burkholderia pyrrocinia
1.11.1.B2 H2O2 above 50 mM Burkholderia pyrrocinia
1.11.1.B2 KCN 0.01 mM, 50% inhibition Burkholderia pyrrocinia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.B2 4.7
-
 H2O2
-
 Burkholderia pyrrocinia
1.11.1.B2 30
-
 Br-
-
 Burkholderia pyrrocinia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.B2 32000
-
 2 * 32000, SDS-PAGE Burkholderia pyrrocinia
1.11.1.B2 64000
-
-
 Burkholderia pyrrocinia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.B2 4-(2-amino-3-chlorophenyl)pyrrole + Cl- + H2O2 Burkholderia pyrrocinia enzyme is involved in the biosynthesis of the antibiotic pyrrolnitrin aminopyrrolnitrin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B2 Burkholderia pyrrocinia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.B2
-
 Burkholderia pyrrocinia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.B2 3.24
-
 bromoperoxidase activity Burkholderia pyrrocinia
1.11.1.B2 4.23
-
 chloroperoxidase activity Burkholderia pyrrocinia

Storage Stability

EC Number Storage Stability Organism
1.11.1.B2 4°C, 0.1 M sodium acetate buffer, pH 3.5-5.5, 0.1 M ammonium acetate buffer, pH 6.0-7.0, 0.1 M potassium phosphate buffer, pH 7.0-8.5, stable for over 2 months Burkholderia pyrrocinia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B2 4-(2-amino-3-chlorophenyl)pyrrole + Cl- + H2O2
-
 Burkholderia pyrrocinia aminopyrrolnitrin + ?
-
 ?
1.11.1.B2 4-(2-amino-3-chlorophenyl)pyrrole + Cl- + H2O2 enzyme is involved in the biosynthesis of the antibiotic pyrrolnitrin Burkholderia pyrrocinia aminopyrrolnitrin + ?
-
 ?
1.11.1.B2 indole + Cl- + H2O2
-
 Burkholderia pyrrocinia oxindole + monochloroindole + H2O
-
 ?
1.11.1.B2 monochlorodimedon + Cl- + H2O2 no activity Burkholderia pyrrocinia dichlorodimedon + H2O
-
 ?
1.11.1.B2 monochlorodimedone + Br- + H2O2
-
 Burkholderia pyrrocinia ?
-
 ?
1.11.1.B2 additional information no peroxidase activity, no catalase activity Burkholderia pyrrocinia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.B2 dimer 2 * 32000, SDS-PAGE Burkholderia pyrrocinia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.B2 60
-
-
 Burkholderia pyrrocinia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.B2 60
-
 2 h, stable Burkholderia pyrrocinia
1.11.1.B2 70
-
 10 min, 50% loss of activity Burkholderia pyrrocinia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.B2 4 4.5
-
 Burkholderia pyrrocinia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.B2 3.5 8.5 stable Burkholderia pyrrocinia

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.B2 additional information non-heme chloroperoxidase Burkholderia pyrrocinia