EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.14 | (+)-muconolactone | - |
Rhodococcus rhodochrous | |
5.4.99.14 | (-)1-Methyl-8-bromobislactone | weak | Rhodococcus rhodochrous | |
5.4.99.14 | 2-Bromo-4-methylbutan-1,4-olide | weak | Rhodococcus rhodochrous | |
5.4.99.14 | 2-Bromobutan-1,4-olide | - |
Rhodococcus rhodochrous | |
5.4.99.14 | 2-Methylbutan-1,4-olide | - |
Rhodococcus rhodochrous | |
5.4.99.14 | 4-Methylbut-3-en-1,4-olide | angelicalactone | Rhodococcus rhodochrous | |
5.4.99.14 | 4-methylbutan-1,4-olide | - |
Rhodococcus rhodochrous | |
5.4.99.14 | AgNO3 | - |
Rhodococcus rhodochrous | |
5.4.99.14 | CuSO4 | - |
Rhodococcus rhodochrous | |
5.4.99.14 | HgCl2 | - |
Rhodococcus rhodochrous | |
5.4.99.14 | additional information | EDTA | Rhodococcus rhodochrous | |
5.4.99.14 | PCMB | - |
Rhodococcus rhodochrous |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.14 | 0.17 | - |
1-methyl-bislactone | - |
Rhodococcus rhodochrous | |
5.4.99.14 | 0.23 | - |
4-carboxymethyl-4-methylbut-2-en-1,4-olide | - |
Rhodococcus rhodochrous |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.4.99.14 | soluble | - |
Rhodococcus rhodochrous | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.14 | 17000 | - |
4 * 17000, Rhodococcus rhodochrous, SDS-PAGE | Rhodococcus rhodochrous |
5.4.99.14 | 75500 | - |
Rhodococcus rhodochrous, gel filtration | Rhodococcus rhodochrous |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.14 | Rhodococcus rhodochrous | - |
N75 | - |
5.4.99.14 | Rhodococcus rhodochrous N75 | - |
N75 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.14 | N75 | Rhodococcus rhodochrous |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.4.99.14 | 4-Carboxymethyl-4-methylbut-2-en-1,4-olide = 4-carboxymethyl-3-methylbut-2-en-1,4-olide | isomerization does not involve actual methyl migration, but proceeds via the 1-methylbislactone | Rhodococcus rhodochrous |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.4.99.14 | culture condition:p-toluate-grown cell | - |
Rhodococcus rhodochrous | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.99.14 | 785 | - |
- |
Rhodococcus rhodochrous |
EC Number | Storage Stability | Organism |
---|---|---|
5.4.99.14 | -30°C, slow loss of activity on prolonged storage | Rhodococcus rhodochrous |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.14 | 1-Methylbislactone | - |
Rhodococcus rhodochrous | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | - |
? | |
5.4.99.14 | 1-Methylbislactone | - |
Rhodococcus rhodochrous N75 | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | - |
? | |
5.4.99.14 | 4-Carboxymethyl-4-methylbut-2-en-1,4-olide | highly specific, accepts only (+)-(4S)-4-methylmuconolactone, i.e. 4-carboxymethyl-4-methylbut-2-en-1,4-olide and the putative reaction intermediate 1-methylbislactone | Rhodococcus rhodochrous | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | i.e. (-)-(4S)-3-methylmuconolactone | ? | |
5.4.99.14 | 4-Carboxymethyl-4-methylbut-2-en-1,4-olide | highly specific, accepts only (+)-(4S)-4-methylmuconolactone, i.e. 4-carboxymethyl-4-methylbut-2-en-1,4-olide and the putative reaction intermediate 1-methylbislactone | Rhodococcus rhodochrous N75 | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | i.e. (-)-(4S)-3-methylmuconolactone | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.14 | tetramer | 4 * 17000, Rhodococcus rhodochrous, SDS-PAGE | Rhodococcus rhodochrous |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.14 | 30 | - |
assay at | Rhodococcus rhodochrous |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.14 | 7 | - |
- |
Rhodococcus rhodochrous |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.14 | 6 | 8.5 | active in this range, rapid decrease of activity above pH 8.5 | Rhodococcus rhodochrous |