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Literature summary extracted from

  • Lederer, F.
    On some aspects of the catalysis of lactate dehydrogenation by flavocytochrome c (1991), Flavins and Flavoproteins (Proc. Int. Symp. , 10th, Meeting Date 1990, Curti, B. , Ronchi S. , Zanetti, G. , eds. ) de Gruyter, Berlin, New York, , 773-782.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3
-
 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.2.3
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 R376K mutant enzyme shows no activity Saccharomyces cerevisiae
1.1.2.3 Y254F significantly reduced activity of mutant enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 0.084
-
 Phenyllactate Y254F mutant Saccharomyces cerevisiae
1.1.2.3 0.35
-
 L-lactate Y254F mutant Saccharomyces cerevisiae
1.1.2.3 0.4
-
 Phenyllactate wild type enzyme Saccharomyces cerevisiae
1.1.2.3 0.49
-
 L-lactate wild type enzyme Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?
1.1.2.3 phenyllactate + ferricytochrome c
-
 Saccharomyces cerevisiae phenylpyruvate + ferrocytochrome c
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.3 0.29
-
 Phenyllactate Y254F mutant enzyme Saccharomyces cerevisiae
1.1.2.3 6.1
-
 L-lactate Y254F mutant enzyme Saccharomyces cerevisiae
1.1.2.3 16.5
-
 Phenyllactate wild type enzyme Saccharomyces cerevisiae
1.1.2.3 270
-
 L-lactate wild type enzyme Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 FMN
-
 Saccharomyces cerevisiae
1.1.2.3 heme
-
 Saccharomyces cerevisiae
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae